159,617 results

1. TFORF0096

   Plasmid

   #142459

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBiT3.1-N_FBXW7:1-707

   Plasmid

   #211099

   Purpose

   Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRBV20-1

   Plasmid

   #123454

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV20-1 (TRBV20-1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-BRD3

   Plasmid

   #65377

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD3 (BRD3 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDECKO_Malat1_A

   Plasmid

   #72620

   Purpose

   Expresses two gRNAs targeting MALAT1 promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward Malat1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEXtev-HMG54kd

   Plasmid

   #26030

   Depositor

   Johann Deisenhofer

   Article

   Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

   Insert

   3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Catalytic domain (amino acids 426-488) Methion…

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET11a-Z-NspGFP

   Plasmid

   #40729

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   N super positive GFP - Leucine Zipper
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Promoter

   Available since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB_TRE3G_scFV-sfGFP

   Plasmid

   #235573

   Purpose

   Dox-inducible expression of control superfolder (sf)GFP fused with scFV

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   superfolder (sf)fGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-RSV-GCaMP6s

    Plasmid

    #80146

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    RSV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ-CBE-Cas9n-Act3.0

    Plasmid

    #178955

    Purpose

    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rat NCX1.1 (pcDNA3.1+)

    Plasmid

    #75190

    Purpose

    Mammalian expression of SLC8A1

    Depositor

    Jonathan Lytton

    Article

    Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

    Insert

    SLC8A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCL-c-MNDU3-X

    Plasmid

    #81071

    Purpose

    Empty lentiviral backbone with the MNDU3 promoter

    Depositor

    Donald Kohn

    Article

    Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30ax

    Plasmid

    #85761

    Purpose

    A bacterial expression vector with a His-tag at N and C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Use

    Tags

    His tag (N-term and C-term)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEB3-mScarlet3_N1

    Plasmid

    #189770

    Purpose

    Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    EB3-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pShuttle-mRFP-gsk3 s9a

    Plasmid

    #24371

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    GSK3beta(S9A) (GSK3B Human)
    

    Use

    Adenoviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S9A. Mutant version of GSK3 that lacks the inhibi…

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL Hygro-NMYC TRF2

    Plasmid

    #16348

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    aa2-500

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

19. M13cp-dg3 hp

    Plasmid

    #218095

    Purpose

    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

    Depositor

    Kevin Esvelt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pN1-CMV-mCherryN-IntN-DrBphP (N23)

    Plasmid

    #210724

    Purpose

    NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    N23
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge