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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVP073

Plasmid

#222076

Purpose

eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pVP074

Plasmid

#222077

Purpose

eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pVP075

Plasmid

#222078

Purpose

AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCS Omomyc

Plasmid

#113164

Purpose

Omomyc expression in mammalian cells

Depositor

Sergio Nasi

Article

Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

Insert

Omomyc (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 WT-pcDNA3.1

Plasmid

#118209

Purpose

Expresses FLAG-OTUB1 WT in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7-W

Plasmid

#159292

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 (KAT7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10)

Promoter

human EF1a promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PC

Plasmid

#92125

Purpose

Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Tags

Expression

Mutation

Promoter

Ppdc

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF1244

Plasmid

#143777

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PHF20 (PHF20 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT3

Plasmid

#60040

Purpose

Mammalian expression of FLAG-tagged mouse TUT3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT3 (Papd5 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
PB6K-mcherry-iATPsnFR1.1

Plasmid

#227239

Purpose

Inducible ATP sensor for Pseudomonas putida

Depositor

Fuzhong Zhang

Article

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiHAscFv-TM

Plasmid

#198302

Purpose

Displays anti-HA scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiHAscFv-TM

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-REL

Plasmid

#27253

Depositor

Thomas Gilmore

Article

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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GZnP3 in pcDNA3.1+

Plasmid

#161738

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

Insert

GZnP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET32-MCM2/7

Plasmid

#116949

Purpose

Expresses human MCM2 and human MCM7

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM7 (MCM7 Human)
Human MCM2 (MCM2 Human)

Use

Tags

Thioredoxin, His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VPS4-E228Q (VPS4A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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