We narrowed to 14,814 results for: NTS;

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pCAG coca-5HT3-IRES-GFP

Plasmid

#242193

Purpose

Expression vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-5HT3-IRES-GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

CAG

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW281_dSpRY

Plasmid

#244822

Purpose

Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

Plasmid

#244835

Purpose

Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Tau BA 12->10 N296N

Plasmid

#203383

Purpose

For transfection and in vitro assay

Depositor

Stefan Stamm

Article

Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

Insert

MAPT (MAPT Human)

Expression

Mammalian

Mutation

N296N (silent mutation)

Promoter

CMVf

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-TDP43HP

Plasmid

#237669

Purpose

For overexpression of mEGFP-NPM1-TDP43HP

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-TDP43HP (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1

Plasmid

#237661

Purpose

For integration of EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS

Plasmid

#237662

Purpose

For integration of EGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-FtoA

Plasmid

#237664

Purpose

For integration of EGFP-NPM1-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoA (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-0F

Plasmid

#237665

Purpose

For integration of EGFP-NPM1-KS-0F

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-0F (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-KS

Plasmid

#237668

Purpose

For overexpression of mEGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-FtoG

Plasmid

#237663

Purpose

For integration of EGFP-NPM1-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoG (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Expression

Plant

Promoter

35S and None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203219

Purpose

Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-QNZF-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

Plasmid

#233038

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233032

Purpose

To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx and mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_N119I_Barcode

Plasmid

#170971

Purpose

Overexpression of RUNX1 N119I mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N119I

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_A120D_Barcode

Plasmid

#170972

Purpose

Overexpression of RUNX1 A120D mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

A120D

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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