We narrowed to 9,745 results for: Tec

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pPU-huMDA5-miR30-L1221

Plasmid

#174223

Purpose

All-in-one huMDA5 rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPKTG

Plasmid

#221911

Purpose

Transient expression of GFP-tagged CBPKTG in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation of lysine residues to glycine residues (…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdCFIDdAL

Plasmid

#221916

Purpose

Transient expression of GFP-tagged CBPdeltaCFIDdeltaAL in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of the CBP-FUS interaction domain (CFID)…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-RATA

Plasmid

#184270

Purpose

Expresses shRNA resistant GFP-RepoMan RATA Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

3xHA and GFP

Expression

Mammalian

Mutation

V383A, F395A

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003(K31TAG)-Talin rod-mScarletI

Plasmid

#210022

Purpose

Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI

Tags

C-terminal mScarletI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-3UTR

Plasmid

#202550

Purpose

Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media

Depositor

Rostislav Skrabana

Article

Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.

Type

Empty backbone

Tags

ATG initiation codon followed by an IGKV3 signal …

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-gephyrin S268E/270E

Plasmid

#199609

Purpose

Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

S268E/S270E

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBVboostFGII WPRE 3C-CVB3

Plasmid

#202077

Purpose

Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB3 3C protease with HisTag

Tags

6xHis-tag

Expression

Bacterial, Insect, and Mamm…

Promoter

polh, CAG, T7

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc

Plasmid

#200127

Purpose

Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB

Plasmid

#148373

Purpose

Insect Expression of Dm4E-T-YLFWtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-YLFWtoAADD (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations H611R and N659S and 8 si…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-rGBD in pmCherryN1

Plasmid

#187286

Purpose

Express IL2-Rhotekin GTPase-binding domain-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Mouse, Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

Plasmid

#169597

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9 - VP64 fusion

Expression

Mammalian

Promoter

PhCMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1046-Tier1-PhCMV-4xCox8-mRuby2

Plasmid

#169537

Purpose

Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir374b~421-CMV2-mir1

Plasmid

#164674

Purpose

Express miR-374b~421 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-374b~421 (MIR374B Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir181b-1~125-CMV2-mir1

Plasmid

#164671

Purpose

Express miR-181b-1~125a and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-181b-1~125a (MIR181B1 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95D293A

Plasmid

#159099

Purpose

CckA FRET Sensor with DITE motif mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Mutation

CckA D293 mutated to A293 and V199I on mRuby3 (P…

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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