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We narrowed to 10,556 results for: aav

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Showing: 9301 - 9320 of 10556 results

  4. pX330-PITCh-TUBB4B

    Plasmid
    #207785
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207785-plasmid-map-sequence-id-416183

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  5. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

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  6. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

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  7. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  10. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid
    #183876
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ANLN sgRNA spacer (ANLN Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    183876-plasmid-map-sequence-id-390709

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  11. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid
    #148859
    Purpose
    Mammalian Expression of HsXRN1-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsXRN1-sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148859-plasmid-map-sequence-id-478935

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  12. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

    Plasmid
    #148855
    Purpose
    Mammalian Expression of HsAGO3-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsAGO3-sgRNA (AGO3 S.pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148855-plasmid-map-sequence-id-401013

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  13. HiFi iFE

    Plasmid
    #190141
    Purpose
    Expresses HiFiCas9-POLB (HiFi iFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9-POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190141-plasmid-map-sequence-id-377886

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  14. SpCas9 dFE

    Plasmid
    #190137
    Purpose
    Expresses SpCas9-T4pol (dFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-T4pol (dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190137-plasmid-map-sequence-id-382840

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  16. pX459-sgRNA1-CTCF-prom

    Plasmid
    #195103
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against CTCF promoter
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195103-plasmid-map-sequence-id-383875

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Showing: 9301 - 9320 of 10556 results