We narrowed to 24,928 results for: crispr
-
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlanineAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL2013
Plasmid#172182PurposeA CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.DepositorInsertmCherry
UseCRISPRExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR
Plasmid#154826PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmCherry
NeoR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianPromoterCMV, EF1a, and SV40 early promoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW FLEx mCherry-KASH
Plasmid#139652PurposeConditional expression of mCherry-KASHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-KASH
ExpressionMammalianPromoterSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
px458_2A_GFP_sgRNA_G3BP1_G1
Plasmid#127114DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA G3BP1 (G3BP1 Human)
UseCRISPRAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRExpressionInsectMutationEGFP-D102G see depositor comments belowAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-Cas9n-UGI
Plasmid#119135PurposeBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restrictionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPOBEC3B L1 intron-Cas9 nickase-UGI
UseCRISPRMutationL1 intron insertionPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CP1012-ABEmax
Plasmid#119806PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)
ExpressionMammalianAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P29
Plasmid#120040PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3C-BE3
Plasmid#113412PurposeExpresses hA3C-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3C-BE3 (APOBEC3C Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3H-BE3
Plasmid#113416PurposeExpresses hA3H-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mA3-BE3
Plasmid#113419PurposeExpresses mA3-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmA3-BE3 (Apobec3 Mouse, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0108
Plasmid#91001PurposeModule A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtCas9
UseCRISPRPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1pr_v1-RT
Plasmid#97087PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear paraspeckle assembly transcript 1 (NEAT1 Human)
UseCRISPRTagsEYFPPromoterCMVAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
px-458-sgRNA-scramble
Plasmid#206924PurposePlasmid expressing Cas9, GFP and non-targeting guides for using as a control in CRISPR experimentsDepositorInsertnon-targeting human sgRNA guides
UseCRISPRPromoterU6Available sinceJan. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only