We narrowed to 12,963 results for: 112
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Plasmid#42045DepositorInsertTNRC6A (TNRC6A Human)
TagsFLAG, HA, and SBPExpressionMammalianMutationR930A, R931A, R932A, R934AAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX302 IL10RA-V5 puro
Plasmid#47552PurposeLentiviral expression of V5-tagged IL10RA in mammalian cellsDepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
TagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-JNKAR
Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AktAR
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176625PurposeTF A self-activation cassette in MultiFate-2.1DepositorInsertTRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GLG1-2Strep
Plasmid#210362PurposeExpresses GLG1 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
DT049A
Plasmid#159410PurposeConstitutive murine mature BDNF expression, controlled under E.coli tet promoterDepositorInsertpr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertet promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-FBL
Plasmid#179245Purposeprotein purification in E. coliDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialPromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast
Plasmid#222634PurposeRho reporter, sensor deadDepositorInsertRhoB Sensor (PKN-L59Q)
UseLentiviralMutationPKN L59QPromoterEF1aAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP1
Plasmid#75163PurposeLentiCRISPR-EGFP with sgRNA targeting human RIPk1DepositorInsertRIPK1 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMflET-o4
Plasmid#101314PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4169
Plasmid#200108PurposePrig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFPDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
TagsmRuby2 and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-TTlight-4xDQNAT
Plasmid#128402PurposePeriplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertTetanus toxin light chain domain
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating E234A mutationPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1
Plasmid#176537PurposeNon-protein coding locus components from KraIscB-1DepositorInsertnon-coding locus components from KraIscB-1 locus
ExpressionBacterialAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-NES-mut
Plasmid#42001DepositorInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationF951A, F955A, I958A, F960APromoterCMVAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-MCART6
Plasmid#133251PurposeExpress FLAG-tagged MCART6 in mammalian cellsDepositorInsertMCART6 (SLC25A53 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only