We narrowed to 9,596 results for: Pol
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Plasmid#240649PurposeThe nucleotides coding 10 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.DepositorInsertUDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)
Tags3xHAExpressionMammalianPromoterCAGAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L328D (316-366)(C-Cys)
Plasmid#193035PurposeBacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.DepositorInsertIST1 (IST1 Human)
Tags6xHis-SumoExpressionBacterialMutationL328D, Residues 316-366, Non-native C-terminal Cy…PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L355A (316-366)(C-Cys)
Plasmid#193036PurposeBacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.DepositorInsertIST1 (IST1 Human)
Tags6xHis-SumoExpressionBacterialMutationL355A, Residues 316-366, Non-native C-terminal Cy…PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiBIRC6-1
Plasmid#202447PurposeExpression of a CRISPRi guide targeting BIRC6DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2(3)-C911
Plasmid#202452Purposea seed-matched control for pRSITEP-puro-shBIRC6-2(3)DepositorAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2(3)
Plasmid#202451PurposeDOX-inducible expression of a short-hairpin RNA targetinging human BIRC6DepositorAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-1
Plasmid#202437PurposeExpression of a guide RNA targeting BIRC6DepositorAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-shBIRC6-2-C911
Plasmid#202450Purposea seed-matched control for pRSITEP-puro-shBIRC6-2DepositorAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiBIRC6-5
Plasmid#202448PurposeExpression of a CRISPRi guide targeting BIRC6DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-4
Plasmid#202438PurposeExpression of a guide RNA targeting BIRC6DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgBIRC6-5
Plasmid#202439PurposeExpression of a guide RNA targeting BIRC6DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
TagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVD1 tRNA-gRNA E4-En-1 (GB2243)
Plasmid#160565PurposetRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInserttRNA-gRNA position E4-En-1 (Multiplexing Edit)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA targeting human SLC12A5 gene stop codon
Plasmid#158577PurposeFor generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInsertsgRNA targeting human SLC12A5 gene stop codon
UseCRISPRTagsCas9 and GFPExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPos-Ma-CmR-TAG111
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
ExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 R62D actin-3XNLS P2A mCherry
Plasmid#58477PurposeExpresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsTagsmCherryExpressionMammalianMutationChanged Arginine 62 to Aspartic AcidPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-RPS6KC1
Plasmid#23460DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145
Plasmid#111731PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ145 (polyQ repeat)PromoterCMV and P10Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only