We narrowed to 12,628 results for: 109

1. pR6Kg-Alpha1spm

   Plasmid

   #165866

   Purpose

   Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-DEST42-SARA-V5-His6

   Plasmid

   #67431

   Purpose

   Expression of hSara with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. GTU-g

   Plasmid

   #81094

   Purpose

   Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   sfGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-hHDAC4

   Plasmid

   #21096

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   Histone Deacetylase 4 (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA target sequence was mutated (mutated nucleo…

   Promoter

   CAG

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pLJM60 FLAG NUFIP1 W40A

   Plasmid

   #110951

   Purpose

   expresses mammalian FLAG-NUFIP1 W40A

   Depositor

   David Sabatini

   Article

   Wyant et al Science. 2018 May 18;360(6390):751-758.

   Insert

   NUFIP1 W40A (NUFIP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Tryptophan 40 to alanine

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-MCPIP1(150-453)

   Plasmid

   #90109

   Purpose

   Mammalian expression of Flag-MCPIP1(150-453)

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pR6Kg-Omega2

   Plasmid

   #165865

   Purpose

   Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pN1-mCherry-NKD2

   Plasmid

   #176641

   Purpose

   Expresses NKD2-mCherry fusion protein in mammalian cells

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   mCherry tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcold-6xHis-HRV3Csite-DmLoqsPD

   Plasmid

   #41095

   Depositor

   Phillip Zamore

   Article

   Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

   Insert

   Loquacious-PD (loqs Fly)
   

   Use

   Tags

   6xHis-HRV3Csite

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pG418R-GMRWhite

    Plasmid

    #165883

    Purpose

    G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcold-6xHis-HRV3Csite-DmLoqsPB

    Plasmid

    #41094

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PB (loqs Fly)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

    Plasmid

    #163610

    Purpose

    Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pNM3746

    Plasmid

    #154109

    Purpose

    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-ARL5B

    Plasmid

    #67379

    Purpose

    Expression of native hArl5b in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL5B (ARL5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGEX6-Smad3 EPSM A208S

    Plasmid

    #27109

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T179V S204A S208A S213A; A208S

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDEST40-SARA-V5-His6

    Plasmid

    #67452

    Purpose

    Mammalian expression of hSara with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221-SARA

    Plasmid

    #67346

    Purpose

    hSara with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)

    Plasmid

    #206462

    Purpose

    Oskar 240-387 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 240-387 (osk Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIPZ-shWT1_A

    Plasmid

    #217636

    Purpose

    Dox inducible shRNA targeting WT-1

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    shWT1_A (WT1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTRIPZ-shWT1_B

    Plasmid

    #217637

    Purpose

    Dox inducible shRNA targeting WT-1

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    shWT1_B (WT1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Halo-MDC1 PST deletion

    Plasmid

    #207109

    Purpose

    pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag-MDC1 PST deletion
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

    Plasmid

    #213398

    Purpose

    transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

    Depositor

    Katrin Willig

    Article

    Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

    Insert

    FingR for endogenous PSD95, transcriptinally regulated
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EGFP-MYO10ΔF3

    Plasmid

    #194857

    Purpose

    Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 ΔF3 (MYO10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncated MYO10 construct. MYO10 amino acids 1-1…

    Promoter

    CMV

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-N1-AAGAB

    Plasmid

    #198333

    Purpose

    Expresses AAGAB-GFP fusion in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. etnppl-pGEMtEasy

    Plasmid

    #192263

    Purpose

    Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

    Depositor

    Toshihide Yamashita

    Article

    Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

    Insert

    ethanolamine phosphate phospholyase (Etnppl Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-15b(ETNPPL)

    Plasmid

    #192264

    Purpose

    Coding sequence of mouse ETNPPL is inserted in pET-15b vector

    Depositor

    Toshihide Yamashita

    Article

    Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

    Insert

    ethanolamine phosphate phospholyase (Etnppl Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEY97

    Plasmid

    #191090

    Purpose

    gpa-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gpa-6 (gpa-6 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEY98

    Plasmid

    #191091

    Purpose

    gpc-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gpc-1 (gpc-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pN1mcherry-NKD2S31A

    Plasmid

    #174332

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-4T Syt1 C2AB(140) M173C

    Plasmid

    #134696

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    M173C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-4T Syt1 C2AB(140) F234C

    Plasmid

    #134697

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F234C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T Syt1 C2AB(140) I367C

    Plasmid

    #134699

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I367C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3.1-NKD2S31A

    Plasmid

    #174331

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    MycHis

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. P[acman]-Alpha1

    Plasmid

    #165858

    Purpose

    Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge