We narrowed to 17,080 results for: EMB

1. pX458-sgRNA_Ago1_1

   Plasmid

   #73533

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-sgRNA_Ago1_2

   Plasmid

   #73534

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHisAlix deltaPRD

   Plasmid

   #42577

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
   

   Tags

   HIS-TEV

   Expression

   Bacterial

   Mutation

   silent mutation A1617G (numbering in ALIX gene)

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pX458-sgRNA_Ago2_3

   Plasmid

   #73531

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bin1 delta SH3

   Plasmid

   #168283

   Purpose

   Express SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.

   Depositor

   Cheng-han Yu

   Article

   Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

   Insert

   Bin1 (Bin1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-404

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-sgRNA_Ago2_4

   Plasmid

   #73532

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CFF-EGFP

   Plasmid

   #86061

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains luminal and transmembrane domains of CD8…

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FB027

   Plasmid

   #119712

   Purpose

   Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
   

   Use

   Synthetic Biology

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEFU6-EGFP_shPKA-Cα

    Plasmid

    #114142

    Purpose

    Expression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    shRNA expression for knock down mouse PKA-Cα
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-sgRNA_Ago1_3

    Plasmid

    #73535

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

12. FB012

    Plasmid

    #119709

    Purpose

    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC:hph:Ttub
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP Gamma 10

    Plasmid

    #36108

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 10 (Gng10 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

    Plasmid

    #207025

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCUP1-GFP-Osh1-406

    Plasmid

    #207007

    Purpose

    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    OSH1
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

    Plasmid

    #207050

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    P85L P86L

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

    Plasmid

    #207051

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    T56A R65A T87A

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

    Plasmid

    #207052

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85A P86A

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

    Plasmid

    #207053

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85L P86L

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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