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pPHR-mCherry-FUSN-mHP1alpha

Plasmid

#181892

Purpose

Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mustard Weed, Mouse)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-TagBFP

Plasmid

#117786

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-FUS-Citrine-PixE

Plasmid

#111506

Purpose

For the nuclear localization of the PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine, FUS, and NLS

Expression

Mutation

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-eGFP

Plasmid

#117785

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-mRFP

Plasmid

#117784

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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UB061 (FCK-stGtACR2-fusionRed)

Plasmid

#135765

Purpose

expresses soma targeted GtACR2

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

stGtACR2

Use

Lentiviral

Tags

Fusion Red

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN-VP16

Plasmid

#183933

Purpose

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-TagBFP

Plasmid

#117783

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Left TALE

Plasmid

#196496

Purpose

Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Right TALE

Plasmid

#196497

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-1-413aa

Plasmid

#29632

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-413

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-H517Q-YFP

Plasmid

#29620

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

H517Q

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R521H-YFP

Plasmid

#29624

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R521H

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-501aa-YFP

Plasmid

#29601

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-501aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-501

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-373aa-YFP

Plasmid

#29598

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-373

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Promoter

Available since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
AAV-CMV-H2B-FusionRed

Plasmid

#164430

Purpose

AAV plasmid expressing nuclear-localized FusionRed fluorophore

Depositor

Connie Cepko

Article

Wang et al Mol Ther Methods Clin Dev. 2020 Oct 10;1

Insert

H2B-FusionRed

Use

AAV

Tags

H2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2022

Availability

Academic Institutions and Nonprofits only

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