We narrowed to 8,199 results for: Gal

1. pCRISPEY-GAL-Nat-ADE2

   Plasmid

   #232103

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-KS-attB2-SA(0)-T2A-Gal4

   Plasmid

   #62899

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS315e_pGal-nCas9(D10A)-PmCDA1

   Plasmid

   #79617

   Purpose

   Expresses nCas9(D10A)-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-hSyn-Galpha-s ONE-GO

   Plasmid

   #214371

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-s ONE-GO (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Feb. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLVX-hSyn-Galpha-q ONE-GO

   Plasmid

   #204336

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-q ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLVX-hSyn-Galpha-13 ONE-GO

   Plasmid

   #204337

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-13 ONE-GO (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

   Plasmid

   #160607

   Purpose

   TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

   Depositor

   Diego Orzaez

   Insert

   CUP2Gal4AD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 416Gal-FUS-P525L-YFP

   Plasmid

   #29628

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   P525L

   Promoter

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

    Plasmid

    #58438

    Purpose

    Expresses LexA-ER-Gal4AD from yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-Gal4AD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gal4-HA-HIF1α(530-652)

    Plasmid

    #24887

    Depositor

    William Kaelin

    Article

    Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

    Insert

    hypoxia inducible factor1alpha (HIF1A Human)
    

    Use

    Tags

    Gal4dbd and HA-tag

    Expression

    Mammalian

    Mutation

    partial HIF1alpha cDNA (encoding aa 530-652)fused…

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdEasy 2-GFP beta-gal

    Plasmid

    #16407

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    acta1

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-kanMX6-PGAL1-3HA

    Plasmid

    #41608

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. ss-SNAP-TMD-NS3-Gal4

    Plasmid

    #112279

    Purpose

    Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    SNAP-TMD-NS3-Gal4
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-FLAG-APEX2-LGALS1

    Plasmid

    #175755

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS1 (LGALS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pKJ1267 (aka pAC-alphaGal4)

    Plasmid

    #21869

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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