Skip to main content
Addgene
Showing: 921 - 940 of 1213 results

  1. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

    Plasmid
    #193037
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, L355A, Residues 316-366, Non-native C-term…
    Promoter
    T7
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193037-plasmid-map-sequence-id-379006

    Enlarge

  2. pCA528 CHMP1B 143-199

    Plasmid
    #108255
    Purpose
    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP1B residues 143-199 (CHMP1B Human)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeli…
    Promoter
    T7
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    108255-plasmid-map-sequence-id-245252

    Enlarge

  3. pCA528 CHMP4B 156-224

    Plasmid
    #104596
    Purpose
    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4B residues 156-224 (CHMP4B Human)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeli…
    Promoter
    T7
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    104596-plasmid-map-sequence-id-245148

    Enlarge

  4. pSMT3-FBF2 SS/Y

    Plasmid
    #166973
    Purpose
    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166973-plasmid-map-sequence-id-331634

    Enlarge

  8. pBJ2

    Plasmid
    #167131
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167131-plasmid-map-sequence-id-330437

    Enlarge

  9. pBJ4

    Plasmid
    #167133
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167133-plasmid-map-sequence-id-330439

    Enlarge

  10. pBJ3

    Plasmid
    #167132
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Frr promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167132-plasmid-map-sequence-id-330441

    Enlarge

  11. pBJ7

    Plasmid
    #167136
    Purpose
    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167136-plasmid-map-sequence-id-330487

    Enlarge

  12. pBJ1

    Plasmid
    #167130
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Frr promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167130-plasmid-map-sequence-id-330432

    Enlarge

  13. pBJ6

    Plasmid
    #167135
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167135-plasmid-map-sequence-id-330485

    Enlarge

  14. pBJ5

    Plasmid
    #167134
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Frr promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167134-plasmid-map-sequence-id-330484

    Enlarge

Showing: 921 - 940 of 1213 results