We narrowed to 9,088 results for: ef1

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piggybac-EF1-halo-Oct4

Plasmid

#197909

Purpose

single-particle tracking for OCT4

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

pou5f1 (Pou5f1 Mouse)

Tags

Halotag

Expression

Mammalian

Promoter

EF1

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp2x 2A Hygro

Plasmid

#102807

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp2x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen

Plasmid

#184704

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6f

Plasmid

#128315

Purpose

Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1

Insert

GCaMP6f

Use

AAV

Tags

His Tag

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Limk1_pLVX-EF1-Cry2-mCherry

Plasmid

#64368

Purpose

lentiviral expression of Link1 and Cry2-mCherry

Depositor

Matthieu Piel

Insert

Limk1 (LIMK1 Human)

Use

Lentiviral

Tags

Cry2-mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-E2-Crimson-NLS

Plasmid

#125263

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

E2-Crimson-NLS

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmScarlet-mAID

Plasmid

#140617

Purpose

Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-jGCaMP8f-WPRE

Plasmid

#176756

Purpose

AAV-mediated expression of GCaMP8f under the EF1a promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

SgRNA expression system

Use

CRISPR

Promoter

U6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

Plasmid

#160045

Purpose

For expressing the active auxin pump PIN2

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

PIN2

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_Short_V5-GFP

Plasmid

#221497

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-EGFP-WPRE-pA

Plasmid

#37085

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH-TAZ S89A

Plasmid

#179910

Purpose

Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Tags

3X Flag-tag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Promoter

EF1a

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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