We narrowed to 12,747 results for: CAR

1. pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #153536

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   Syn

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-shP53

   Plasmid

   #124261

   Purpose

   Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   shP53
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLVX-Flag-CDK19-WT-IRES-ZsGreen

   Plasmid

   #68858

   Purpose

   Expresses Human CDK19 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65454

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 Sox2 HM a

   Plasmid

   #26353

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT1-2A-eGFP PGK PuroR Donor Plasmid

   Plasmid

   #82333

   Purpose

   WT1 targeting donor

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Inserts

   WT1 5' Arm (WT1 Human)
   WT1 3' Arm (WT1 )
   

   Use

   CRISPR and TALEN

   Tags

   2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

   Plasmid

   #112677

   Purpose

   An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Nuclear-localized floxed-mCherry EGFP
   

   Use

   AAV

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304_zeo_mmFbxw7a

   Plasmid

   #160102

   Purpose

   Expresses murine Fbxw7 alpha in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Fbxw7alpha (Fbxw7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234435

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid

    #61576

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    chromatin insulator flanked TRE-LSL-tdTomato
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer10b-HA-KRAS WT CO SR

    Plasmid

    #164926

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mutation

    9 Valine codons are optimized and siRNA targetin…

    Promoter

    Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ad:2CMV_A2R2-N25Ctr

    Plasmid

    #122243

    Purpose

    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1a-hDNAJC6-T2A-copGFP

    Plasmid

    #170443

    Purpose

    Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human DNAJC6 (DNAJC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFC0996

    Plasmid

    #186695

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-MBP-BrCas12b-FNLDTA

    Plasmid

    #195340

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis Tag and MBP, TEV cleavage site, 6x His Tag

    Expression

    Bacterial

    Mutation

    F208W, N524V, L795I, D868V, T874S, A1015E

    Promoter

    T7 Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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