174,739 results

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs PQR>ala

Plasmid

#83319

Purpose

human DNA-PKcs PQR>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

5 phosphorylation sites in the PQR cluster substi…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EBF1

Plasmid

#96965

Purpose

Dox-inducible expression of Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

Ebf1 (Ebf1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting

Plasmid

#92141

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Human, Synthetic)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87829

Purpose

High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

MTS
NLS
PTS1

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTiger-OptoSOS

Plasmid

#86439

Purpose

Single-construct blue light optogenetic Ras activator for use in Drosophila

Depositor

Jared Toettcher

Article

Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

Insert

OptoSOS

Tags

tagRFP

Expression

Insect

Promoter

Gal4-UAS

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Article

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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CeNL/pcDNA3

Plasmid

#85199

Purpose

Cyan color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Cyan enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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CAG sHRPa-NRX

Plasmid

#73143

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Tags

2x HA tag and Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pUCP20T-tdTomato

Plasmid

#78469

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

tdTomato

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-oG-WPRE-SV40pA

Plasmid

#74292

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

CAG

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC1A3

Plasmid

#72873

Purpose

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

SLC1A3 (SLC1A3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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