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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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177,604 results

Showing: 9481 - 9500 of 177604 results

  9. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Expression
    Mammalian
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    216798-plasmid-map-sequence-id-428831

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  11. pcDNA3.1+-Tornado-nLuc

    Plasmid
    #212611
    Purpose
    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.
    Depositor
    Samie Jaffrey
    Publication
    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tornado-CVB3-nLuc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212611-plasmid-map-sequence-id-420917

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  17. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Publication
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    170069-plasmid-map-sequence-id-333408

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  18. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid
    #185822
    Purpose
    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.
    Depositor
    Atsushi Miyawaki
    Publication
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    er-(n2)oxStayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    185822-plasmid-map-sequence-id-366124

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  19. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    184225-plasmid-map-sequence-id-361604

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Showing: 9481 - 9500 of 177604 results