165,688 results

1. pRK5-HA-YFP-rictor

   Plasmid

   #73387

   Purpose

   Expresses HA and YFP tagged Rictor

   Depositor

   Taekjip Ha, Jie Chen

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

   Plasmid

   #21849

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   N-terminal transactivation domain deleted and rep…

   Available Since

   Sept. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-fSNAP-NAT

   Plasmid

   #188916

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TARDBP_Halo_N_allele

   Plasmid

   #178131

   Purpose

   Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (TARDBP Human)
   

   Use

   Donor vector

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG235

   Plasmid

   #64533

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-NmCas9-Flag

   Plasmid

   #109030

   Purpose

   Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWM_12x601_15bpLinker

   Plasmid

   #157786

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_15bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

    Plasmid

    #226515

    Purpose

    Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    Cox8a(4x)-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJA167

    Plasmid

    #128536

    Purpose

    Expresses DsRed

    Depositor

    Marcos Alcocer

    Article

    Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

    Insert

    NFAT-DsRed reporter
    

    Expression

    Mammalian

    Promoter

    SV40-NFAT

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamin A-mRFP

    Plasmid

    #124268

    Purpose

    Wild-type Lamin A, C-terminally tagged with mRFP

    Depositor

    Eric Schirmer

    Insert

    LMNA (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid

    #61628

    Purpose

    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    ntdTomato
    n2xEGFP
    

    Expression

    Plant

    Mutation

    2 copies of EGFP with N-terminal SV40 NLS

    Promoter

    Auxin-dependent DR5 promoter containing 9 reverse…

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. IGF2BP1

    Plasmid

    #155676

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL29

    Plasmid

    #15457

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    
    

    Use

    S. aureus-e. coli shuttle vector

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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