162,536 results

1. pARS3_MUbCAS9_GW

   Plasmid

   #128646

   Purpose

   T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

   Depositor

   G. Eric Schaller

   Article

   Worthen et al Development. 2019 Jun 3. pii: dev.174870

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. spNW15

   Plasmid

   #173483

   Purpose

   Expresses spNW15 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW15
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFVK5

   Plasmid

   #167522

   Purpose

   Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   VanRAM-mKelly2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PVanCC

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-Pickles 2.34NES

   Plasmid

   #100028

   Purpose

   Improved FRET biosensor for the measurement of BCR-ABL activity.

   Depositor

   Yusuke Ohba

   Article

   Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

   Insert

   Pickles-2.34NES
   

   Use

   Pfx vector is derived from pcmv vector.

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

   Plasmid

   #158167

   Purpose

   Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CAMK1D (CAMK1D Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEVA3b67Rb

   Plasmid

   #58311

   Purpose

   Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PMXS-NDI1

   Plasmid

   #72876

   Purpose

   expresses NDI1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

   Insert

   NDI1 (NDI1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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