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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro p53 shRNA 2

Plasmid

#10672

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pFE-Reu-FI-prk

Plasmid

#162696

Purpose

Expresses R. eutropha Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. eutropha CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Ran(Q69L)

Plasmid

#42048

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Ran (RAN Human)

Tags

His

Expression

Bacterial

Mutation

Q69L

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-WT

Plasmid

#240020

Purpose

Expresses human DDX6 fused to EGFP in mammalian cells

Depositor

Rui Su

Insert

DDX6

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDsRed-monomer-hAIM2

Plasmid

#51544

Purpose

Express DsRed monomer-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

DsRed monomer

Expression

Bacterial

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD wt

Plasmid

#19316

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAGM4673

Plasmid

#48014

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pD40-His/V5-c-MycT58A

Plasmid

#45598

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc T58A (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Mutation

T58A

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/Envelope

Plasmid

#120821

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Envelope ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3

Plasmid

#175778

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn WT

Plasmid

#107425

Purpose

Mammalian expression of human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein (SNCA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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JD150

Plasmid

#229485

Purpose

Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, VA RNA I, E4orf6, L4-22k/33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP1356

Plasmid

#119276

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-GFP mAb

Plasmid

#214955

Purpose

Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-CD63-FKBP-Myc

Plasmid

#177910

Purpose

human CD63-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD63 (CD63 Human)

Tags

FKBP and Myc tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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