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pFUGW Cas9-BFP

Plasmid

#127396

Purpose

Cas9 expression

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Insert

Cas9

Use

Lentiviral

Tags

BFP

Promoter

SVVF

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pJUMP27-1A(sfGFP)

Plasmid

#126974

Purpose

Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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hBax C3-EGFP

Plasmid

#19741

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBIG1D-eIF3bgi

Plasmid

#240040

Purpose

Human eIF3b, eIF3g and eIF3i subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3b, g, i

Tags

eIF3i: HIS10

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7g

Plasmid

#51381

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7g (MIRLET7G Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pBbA6c-RFP

Plasmid

#35290

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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TGT-His

Plasmid

#138201

Purpose

Expresses E. Coli TGT with a C-terminal His Tag

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Insert

E Coli tRNA Guanine Transglycosylase-His

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSMP-Luc

Plasmid

#36394

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Firefly Luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pBbB5k-GFP

Plasmid

#35354

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG G-CEPIA1er

Plasmid

#105012

Purpose

ER calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

G-CEPIA1er

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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P-fusA2

Plasmid

#235656

Purpose

Reports Cur activity in Bacteroides thetaiotaomicron

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pVHed05

Plasmid

#82351

Purpose

Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

TP901-1 integrase
Bxb1 integrase
AraC (araC )
TetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19P

Plasmid

#32631

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

SADB19P

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pBbB5c-GFP

Plasmid

#35353

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE8k-RFP

Plasmid

#35270

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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