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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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HBA1 full 3'UTR-T4 td downstream intron

Plasmid

#189974

Purpose

Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HBA1 full 3'UTR-T4 td downstream intron

Use

Cloning

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9aQ66L

Plasmid

#169072

Purpose

Express human constitutively active form of Rab9a(Q66L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q66L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mCherry

Plasmid

#50462

Purpose

Double floxed mCherry under the control of EF1a promoter

Depositor

Bryan Roth

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

EF1a

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVBEX1-WT-Myc-FLAG

Plasmid

#74506

Purpose

Retroviral expression of BEX1

Depositor

Lars Rönnstrand

Article

Lindblad et al Oncotarget. 2015 Aug 28;6(25):21395-405.

Insert

BEX1 (BEX1 Human)

Use

Retroviral

Tags

FLAG and Myc

Expression

Mammalian

Promoter

unknown

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-EGFP(HiBiT)

Plasmid

#162589

Purpose

N-terminally HiBiT-tagged EGFP under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

EGFP

Tags

HiBiT

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GFP

Plasmid

#175757

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GFP

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pDL

Plasmid

#208978

Purpose

Dual-luciferase plasmid to measure enhancer activity in plants

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2024 Mar 21:koae088. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mCherry_tr

Plasmid

#111549

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSIMcpf1

Plasmid

#153034

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_15

Plasmid

#194676

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_15

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP PPP1R8 G4

Plasmid

#129021

Purpose

Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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