We narrowed to 21,826 results for: Sis

1. pBN084

   Plasmid

   #173446

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y708E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y708E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBN085

   Plasmid

   #173447

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN008

   Plasmid

   #167949

   Purpose

   express E. coli E69 (O9a:K30) Wzc with K540M mutation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBN093

   Plasmid

   #167951

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, delete motif 3

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBN074

   Plasmid

   #167955

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y705E, Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TetO::EGFP-hMeikin(8A mut)

   Plasmid

   #174707

   Purpose

   Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHRSF228

   Plasmid

   #135007

   Purpose

   MusF2 prenyltransferases from Nostoc sp. UHCC 0398

   Depositor

   Hideo Iwai

   Article

   Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

   Insert

   MusF2 prenyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TLCV2-sgHPDL #3

   Plasmid

   #174165

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS426-CeLACE-1

    Plasmid

    #174523

    Purpose

    Express C.elegans LACE-1 in yeast cells

    Depositor

    Oleh Khalimonchuk

    Article

    Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

    Insert

    lace-1 (C30F12.2 Nematode)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL15

    Plasmid

    #174069

    Purpose

    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-U63-tgRNA-Gal80

    Plasmid

    #169030

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ABEmax(TadA E59Q, TadA* V106W)

    Plasmid

    #126496

    Purpose

    Expresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59Q TadA* V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-ABEmax(TadA E59, TadA* V106W)

    Plasmid

    #126497

    Purpose

    Expresses ABEmax(TadA E59, TadA*V106W) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59 TadA* V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge