We narrowed to 50,608 results for: ang

1. pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236362

   Purpose

   Overexpression of HyperdAsCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   E174R, D256R, E321R, E371R

   Promoter

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

   Plasmid

   #236361

   Purpose

   Overexpression of dAsCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   dAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ptet-lux-Tn7T-Gm

   Plasmid

   #226434

   Purpose

   This vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.

   Depositor

   Xue Liu

   Article

   Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:

   Inserts

   optimized TetR
   luxABCDE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcon and Ptet

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21b-effector-ncRNA-rrt-His8

   Plasmid

   #194089

   Purpose

   Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 effector-ncRNA-rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-jGCaMP8s

   Plasmid

   #162371

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBT2-4xUAS:P2A-EGFP

   Plasmid

   #127591

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Lyso-Digda

   Plasmid

   #236102

   Purpose

   Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   Lyso-Digda (LAMP1 Human, Synthetic)
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LV-hNG2-GFP

    Plasmid

    #183910

    Purpose

    Expression of GFP under the human NG2 promoter

    Depositor

    Chun-Li Zhang

    Article

    Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

    Insert

    GFP (GFAP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Tag2B-E2F1

    Plasmid

    #202520

    Purpose

    Expressing human E2F1 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag2B-E2F3

    Plasmid

    #202522

    Purpose

    Expressing human E2F3 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_PGK_antiCD19_eZ3_Gal4VP64

    Plasmid

    #169917

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Additional EGF repeat between the extracellular d…

    Promoter

    PGK

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYLsgRNA-OsU3m/LacZ

    Plasmid

    #66192

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU3m (SpeI site destroyed)

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TOP2A sgRNA2

    Plasmid

    #138191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-Tag2B-E2F2

    Plasmid

    #202521

    Purpose

    Expressing human E2F2 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F2 (E2F2 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRRAP sgRNA1

    Plasmid

    #138182

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRRAP sgRNA3

    Plasmid

    #138184

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TRRAP sgRNA2

    Plasmid

    #138183

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAM4663

    Plasmid

    #85126

    Purpose

    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiB-luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p-miR-Report + WT miR-181 BS in NLK 3UTR

    Plasmid

    #22790

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pAM4731

    Plasmid

    #85127

    Purpose

    NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiBC-KaiB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p-miR-Report + MT miR-181 BS in NLK 3UTR

    Plasmid

    #22791

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pCAG-48xGPBP1 array

    Plasmid

    #236383

    Purpose

    Expression of array of 48 crRNAs targeting GPBP1

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    48xGPBP1 crRNA array (GPBP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236376

    Purpose

    Overexpression of HyperdFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E184R, T279R, D340R, D409R

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-48xHSPA1A array

    Plasmid

    #236377

    Purpose

    Expression of array of 48 crRNAs targeting HSPA1A

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    48xHSPA1A crRNA array (HSPA1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

    Plasmid

    #236368

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-48xH3C1 array

    Plasmid

    #236384

    Purpose

    Expression of array of 48 crRNAs targeting H3C1

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    48xH3C1 crRNA array (H3C1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA6-N-Flag-SREBP2

    Plasmid

    #134284

    Purpose

    Expresses Flag-tagged SREBP2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. tet-pLKO-sgRNA-puro

    Plasmid

    #104321

    Purpose

    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

    Depositor

    Nathanael Gray

    Article

    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA6-N-Myc-SCAP

    Plasmid

    #134285

    Purpose

    Expresses Myc-tagged SCAP in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SCAP (Scap, Scap Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162386

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171380

    Purpose

    Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized RfxCas13d
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162388

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LentiCRISPR v2 RB1 sgRNA #1

    Plasmid

    #202516

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162385

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LentiCRISPR v2 RB1 sgRNA #2

    Plasmid

    #202519

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGL3-ACSL4-FL

    Plasmid

    #202515

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCAG-36XMYC array

    Plasmid

    #236380

    Purpose

    Expression of array of 36 crRNAs targeting MYC

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    36xMYC crRNA array (MYC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCAG-48xEFNA1 array

    Plasmid

    #236382

    Purpose

    Expression of array of 48 crRNAs targeting EFNA1

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    48xEFNA1 crRNA array (EFNA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-Lyso-ExRai-CKAR2

    Plasmid

    #236100

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    LAMP1-ExRai-CKAR2 (LAMP1 Human, Synthetic)
    

    Use

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBT2-4xUAS:NICD-P2A-mRFP

    Plasmid

    #127594

    Purpose

    Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Insert

    NICD (notch1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge