We narrowed to 7,112 results for: ef1a
-
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-mRFP1
Plasmid#112914PurposeRetrovirus expressing mRFP1 with BBSI cloning sites for sgRNADepositorInsertmRFP1
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2
Plasmid#129715PurposeAAVS1 safe harbor targeting vector expressing AtAFB2DepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsnoExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag
Plasmid#132407Purposeoverexpression in human cellsDepositorInsertdLwaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR474A; H479A; R1046A; R1051APromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag
Plasmid#132401Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-IGK-dAPEX2-KDEL
Plasmid#117183PurposePlasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-MSK1
Plasmid#165601PurposeExpresses Sp dCas9 fused to human MSK1DepositorInsertribosomal protein S6 kinase A5 (RPS6KA5 Human, S. Pyogenes, Synthetic)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS133
Plasmid#211504PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmTagBF2
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS134
Plasmid#211505PurposeAAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertVenus
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS132
Plasmid#211503PurposeAAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmCherry
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos MAL Flag
Plasmid#41554DepositorInsertMAL (TIRAP Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJK2243
Plasmid#227688PurposeEmpty lentivector to overexpress gene of interestDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 KRAB
Plasmid#188501PurposeExpresses FLAG tagged SpRY dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX307 hcRed1
Plasmid#117732PurposeOpen reading frame vector encoding hcRed1DepositorInserthcRed1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag
Plasmid#132412Purposeoverexpression in human cellsDepositorInsertdAdmCas13b
UseLentiviralTagsflagExpressionMammalianMutationR269A; H274A; R837A; H842APromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX311 hcRed1
Plasmid#117733PurposeOpen reading frame vector encoding hcRed1DepositorInserthcRed1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC841 - pAAV minP secNLuc
Plasmid#82498PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVTagsExpressionMammalianMutationPromoterc-Fos minimal promoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBG-PELP1
Plasmid#65209PurposeFull length mammalian expression vector for hPELP1DepositorInsertPELP1 (PELP1 Human)
UseTagsGSTExpressionMammalianMutationPromoterEF1aAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmbim6-V5
Plasmid#175150PurposeLentiviral expression of mouse Tmbim6-V5DepositorInsertTmbim6 (Tmbim6 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem9-V5
Plasmid#175151PurposeLentiviral expression of mouse Tmem9-V5DepositorInsertTmem9 (Tmem9 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-IGK-dAPEX2-KDEL
Plasmid#117184PurposePlasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Vkorc1l1
Plasmid#175148PurposeLentiviral expression of V5-tagged mouse Vkorc1l1DepositorInsertVkorc1l1 (Vkorc1l1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD/Bla
Plasmid#201559PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-W/Bla
Plasmid#201558PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc and FLAG-EGFPExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem161a-V5
Plasmid#175149PurposeLentiviral expression of mouse Tmem161a-V5DepositorInsertTmem161a (Tmem161a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem214
Plasmid#175153PurposeLentiviral expression of V5-tagged mouse Tmem214DepositorInsertTmem214 (Tmem214 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K113R-3xFlag_IRES-GFP
Plasmid#198391PurposeRPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site mutant (K113R)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 VPR
Plasmid#188511PurposeExpresses FLAG tagged SpRY dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF HA-TSAdwt
Plasmid#84517PurposeMammalian expression of TSAdDepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoH2B:Venus
Plasmid#111877PurposeThis plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.DepositorInsertCapsaspora Histone H2B (CoH2B) fused to Venus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorInsertTmem53 (Tmem53 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDEF3‐mTim1 (no tag)
Plasmid#49206Purposeexpresses mouse Tim1DepositorInsertTim1 (Havcr1 Mouse)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
rAAV-Ef1α-EGFP-WPRE-ID-PolyA
Plasmid#241829PurposeAAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.DepositorInsertEGFP-WPRE-ID-PolyA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFP-2xNLS
Plasmid#236371PurposeOverexpression of MCP-3XBFP-2xNLS in human cellsDepositorInsertMCP-3XBFP-2xnls
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-Halo-NLS-tDeg
Plasmid#236370PurposeOverexpression of Halo-NLS-tDeg in human cellsDepositorInsertHalo-NLS-tDeg
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)
Plasmid#238185PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Human, Synthetic)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)
Plasmid#238181PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Human, Synthetic)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)
Plasmid#238184PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Human, Synthetic)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)
Plasmid#238182PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Human, Synthetic)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-PTPN12(D199A,C231S)-Clover
Plasmid#215528PurposeExpression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)DepositorInsertPTPN12 (PTPN12 Human)
UseTagsCloverExpressionMammalianMutationD199A, C231S (Phosphatase inactive)PromoterEF1aAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF.DEST51-Dok1-Clover
Plasmid#215456PurposeExpression vector with Dok1 tagged with CloverDepositorInsertDOK1 (DOK1 Human)
UseTagsCloverExpressionMammalianMutationPromoterEF1aAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX552-DIO-NLS-mRuby3(with gRNA scaffold)
Plasmid#199583PurposeExpresses NLS-mRuby3 in a Cre-dependent mannerDepositorInsertNLS-mRuby3
UseAAV, CRISPR, and Cre/LoxTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e VPR
Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Pigb-V5
Plasmid#175160PurposeLentiviral expression of mouse Pigb-V5DepositorInsertPigb (Pigb Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem209
Plasmid#175154PurposeLentiviral expression of V5-tagged mouse Tmem209DepositorInsertTmem209 (Tmem209 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Zdhhc6-V5
Plasmid#175146PurposeLentiviral expression of mouse Zdhhc6-V5DepositorInsertZdhhc6 (Zdhhc6 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only