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Showing: 941 - 960 of 1667 results
  1. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    JNKsub(TA)DD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pToxamEXP4CUP1

    Plasmid
    #218284
    Purpose
    Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus
    Depositor
    Insert
    Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. YW347_Ptbb2UTR-loxP-RFP-miniMOS

    Plasmid
    #196064
    Purpose
    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.
    Depositor
    Inserts
    myo2p driven tagRFP
    NeoR
    Minimal Mos1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    myo-2
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid
    #106309
    Purpose
    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.
    Depositor
    Insert
    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    Use
    Tags
    6xflag-3xHaloTag(MtoL)
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pLJM1-EGFP-GRAM-W

    Plasmid
    #211704
    Purpose
    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)
    Depositor
    Insert
    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 941 - 960 of 1667 results