We narrowed to 21,668 results for: Tes

1. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-elavl3

   Plasmid

   #75025

   Purpose

   Can be used to drive pan neuronal expression in zebrafish.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   elavl3

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-CMV-Luc2-IRES-ZsGreen-W

   Plasmid

   #164432

   Purpose

   Lentiviral vector backbone encoding firefly luciferase and ZsGreen

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Inserts

   Luciferase
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP Abi1

   Plasmid

   #74905

   Purpose

   Expresses human Abi1 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

   Insert

   Abi1 (ABL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Miro1 S156A

   Plasmid

   #127588

   Purpose

   Phospho-mutant of S156

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S156A

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2plus-mKate2

   Plasmid

   #162624

   Purpose

   Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    gfap

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTet-O-ASCL1-T2A-PuroR

    Plasmid

    #162345

    Purpose

    Lentiviral expression of ASCL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTet-O-LHX8-T2A-PuroR

    Plasmid

    #162350

    Purpose

    Lentiviral expression of LHX8 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    LHX8 (LHX8 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32005

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mKate2-P2A-APEX2-TAPP1-PH

    Plasmid

    #67662

    Purpose

    Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-TAPP1-PH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge