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Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET-2HMG-mCherry

Plasmid

#112739

Purpose

Express 2HMG-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al RSC Adv, 2016, 6, 46291-46298

Insert

2HMG-mCherry

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pXYL (J) P3 (SBE108)

Plasmid

#194991

Purpose

promoter of the gene xylose reductase from R. toruloides for a position P3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pXYL (J)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE663

Plasmid

#153261

Purpose

M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE665

Plasmid

#153263

Purpose

M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE654

Plasmid

#153252

Purpose

M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pM2_PLLaco1R1CI*_PLTetO1R1CI*

Plasmid

#250895

Purpose

Cassette 1: Mutated cI protein expressed under PL TetO1 promoter; Cassette 2: Mutated cI protein expressed under PL LacO1 promoter; part of BNeu AS10; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein

Promoter

PL LacO1 and PL TetO1

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLTetO1R2CIw

Plasmid

#250893

Purpose

Cassette 1: Wild type cI protein expressed under PL TetO1, regulated by RBS 'R2'; Cassette 2: Wild type cI protein expressed under PL LacO1 and regulated b RBS 'R3'; part of Bneu AS7; p15A origin

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Wild type cI
Wild type cI

Use

Synthetic Biology

Promoter

PL LacO1 and PL TetO1

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eco1

Plasmid

#240705

Purpose

pLAM12 vector backbone containing Eco1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Eco1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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