We narrowed to 14,744 results for: RING

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox; Gateway cloning

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-mRuby-Rab8a

Plasmid

#194337

Purpose

Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Rab8 (RAB8A Canis lupus)

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pCSDest2-NmeCas9-NLS-3XHA-NLS

Plasmid

#87448

Purpose

Mammalian expression of wild type Nme Cas9

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Nme Cas9

Use

CRISPR

Tags

3XHA, SV40 NLS, and cMyc-like NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-MEKK3

Plasmid

#12189

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

MEKK3 (Map3k3 Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
PAX6 CKO 2/7 Donor plasmid

Plasmid

#68467

Purpose

donor plasmid for targeting PAX6 locus

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

FRT flanked exon4 of PAX6 gene (PAX6 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSR357.10

Plasmid

#125106

Purpose

Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgMUC4

Plasmid

#198399

Purpose

Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

MUC4 sgRNA (MUC4 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAS4.1w-tet-on-NORAD

Plasmid

#125010

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)

Expression

Mammalian

Promoter

TRE-Tight

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-NHA-Ago2

Plasmid

#115361

Purpose

Expression vector to produce NHA-tagged human Ago2 protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

Argonaute 2 (AGO2 Human)

Tags

N peptide and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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