161,123 results

1. pGBScrispri Sham

   Plasmid

   #223201

   Purpose

   Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA

   Depositor

   Thomas Hooven

   Insert

   N/A
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AcGFP1-C1

   Plasmid

   #54607

   Purpose

   Localization: C1 Cloning Vector, Excitation: 475, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_KRAS_WT

   Plasmid

   #81923

   Purpose

   Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-GW-HA-RINT1

   Plasmid

   #39841

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   RINT1 (RINT1 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD112

   Plasmid

   #119893

   Purpose

   pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TNFR1-YFP

   Plasmid

   #111209

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   TNFRSF1A (TNFRSF1A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2_IRES2_EGFP_KCNQ2 var4

   Plasmid

   #173154

   Purpose

   For mammalian expression of KCNQ2 and creating variants

   Depositor

   Al George

   Article

   Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

   Insert

   KCNQ2 (KCNQ2 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Simian CMV IE94

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A-GFP-N2 (FR1)

    Plasmid

    #19364

    Purpose

    Gateway entry vector for C-term eGFP fusion, frame N2

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

    Plasmid

    #224501

    Purpose

    Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin B Vhh mNeonGreen-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

    Plasmid

    #160540

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Inserts

    SARS-CoV-2 NSP7 (ORF1ab Synthetic)
    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    SARS-CoV-2 NSP12 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. WT NFAT1

    Plasmid

    #11100

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-Nanog

    Plasmid

    #13354

    Purpose

    Retroviral expression of mouse Nanog

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVHA E2F1

    Plasmid

    #24225

    Purpose

    Mammalian expression of human E2F1 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFGC5941-PacI-ath-STTM165/166-48nt

    Plasmid

    #84091

    Purpose

    target miRNA165/166 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM165/166
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEYFP-hDF

    Plasmid

    #72584

    Purpose

    human dysferlin full length tagged with YFP

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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