20,081 results

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Article

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pIRES-Venus

Plasmid

#22665

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

IRES-Venus

Expression

Mammalian

Mutation

IRES is EMCV IRES

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-Flex-GFP-Fishell_6

Plasmid

#83895

Purpose

Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

Insert

eGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Tags

Strep II and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p75-RFP

Plasmid

#24092

Depositor

Moses Chao

Insert

p75NTR (NGFR Human)

Tags

DSRed

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSBtet-GH

Plasmid

#60498

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-hINO80

Plasmid

#44149

Depositor

Joan Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

hINO80 full length (INO80 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec23A

Plasmid

#166894

Purpose

mammalian expression of Sec23A tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-EV

Plasmid

#163454

Purpose

empty vector control for pLJC6

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Mtp-p53-ECFP

Plasmid

#53961

Purpose

Expresses CFP tagged p53 in mammalian cells

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

p53 (TP53 Human)

Tags

ECFP

Expression

Mammalian

Promoter

metallothionein promoter

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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M43: pHR-SFFVp-iLID::EGFP::FTH1

Plasmid

#122149

Purpose

Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

NLS-iLID-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-T7-hOsa2

Plasmid

#17987

Depositor

Naoko Tanese

Article

Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

Insert

Osa2 (ARID1B Human)

Tags

T7 epitope

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-RdLight1

Plasmid

#125706

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

RdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4 HisMax-V5-GFP-RRBP1

Plasmid

#92150

Purpose

Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 (RRBP1 Human)

Tags

Calreticulin signal peptide, EGFP (A207K mutation…

Expression

Mammalian

Mutation

In RRBP1: N951S and T1329L. E1279 is also missing.

Promoter

CMV

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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