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Bxb1-GT-Bactin-mStayGold

Plasmid

#241391

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

Bactin

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-eGFP

Plasmid

#242758

Purpose

Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-EGFP

Expression

Bacterial

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-PGK-mStayGold

Plasmid

#241392

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-hEF1a-mStayGold

Plasmid

#241377

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

hEF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-ShortCAG-mStayGold

Plasmid

#241385

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

ShortCAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-PGK-mStayGold

Plasmid

#241384

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-SV40-mStayGold

Plasmid

#241382

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hDNMT3B_KO_puro

Plasmid

#234998

Purpose

Stable knockout of DNMT3B function in mammalian cells

Depositor

Samrat Roy Choudhury

Article

Choudhury et al Genes Dis. 2025 Sep 6;13(1):101843. doi:

Insert

hCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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Scramble_gRNA_puro

Plasmid

#234999

Purpose

scramble sgRNA

Depositor

Samrat Roy Choudhury

Article

Choudhury et al Genes Dis. 2025 Sep 6;13(1):101843. doi:

Insert

scramble sgRNA

Use

Lentiviral

Tags

EGFP:T2A:Puro

Expression

Mammalian

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

Plasmid

#235277

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL-bGH

Plasmid

#235299

Purpose

mGreenLantern expression to serve as a transfection marker, EF1a-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

Plasmid

#235282

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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U6-miR.FF4

Plasmid

#235256

Purpose

Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FF4 hairpin

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

Plasmid

#235260

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRO204

Plasmid

#233843

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO241

Plasmid

#233844

Purpose

MoClo-YTK; ConLS/R1 cassette; ABA systems; pTDH3-Phlf-NES-PYL1-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-Phlf-NES-PYL1-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO279

Plasmid

#233845

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-1xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-1xOaf1ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO280

Plasmid

#233846

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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