We narrowed to 16,130 results for: g418

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pDEST40-2XFL-Src-K298M

Plasmid

#140315

Purpose

Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hTel2

Plasmid

#30214

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

telomere maintenance 2 (TELO2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only
hCEP120-EGFP

Plasmid

#50382

Purpose

Over-expression of EGFP-tagged hCEP120

Depositor

Tang Tang

Article

Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

Insert

Human CEP120 (CEP120 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDM304_DPAKa(GBD)-YFP

Plasmid

#128699

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Mutation

none

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Beta1-GFP pFB-Neo

Plasmid

#69767

Purpose

Expresses Beta1-GFP integrin for retroviral transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1 integrin (ITGB1 Human)

Use

Retroviral

Tags

GFP

Promoter

IRES

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pTrex-Neo-tdTomato

Plasmid

#47975

Purpose

Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

tdTomato

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTOPO-MCL1

Plasmid

#21605

Depositor

Ulrich Maurer

Article

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 (MCL1 Human)

Expression

Mammalian

Mutation

Insert ends with a STOP codon, no V5-His-tag.

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
EHD2-mCherry

Plasmid

#45933

Purpose

caveolar protein, confines caveolae to the plasma membrane

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pUb-Cas9-EGFP_cU6:6-sgRNA

Plasmid

#190597

Purpose

The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

EGFP - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1383

Plasmid

#207423

Purpose

Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
Raf1 (RAF1 Human)

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–134 (RBD) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-SspBx6

Plasmid

#103778

Purpose

expression of iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-SspBx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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EBFP2-Cx30

Plasmid

#69022

Purpose

Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx30 (GJB6 Human)

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NLS

Plasmid

#175007

Purpose

Nuclear-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Clathrin-15

Plasmid

#58005

Purpose

Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PREX2 pcdna3.1 V5/his

Plasmid

#41555

Depositor

Ramon Parsons

Article

Fine et al Science. 2009 Sep 4;325(5945):1261-5.

Insert

PREX2a (PREX2 Human)

Tags

V5 and his

Expression

Mammalian

Promoter

T7

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187916

Purpose

Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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