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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Publication

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-LSSmKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Publication

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Publication

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-N1.ARRDC1

Plasmid

#38320

Depositor

Quan Lu

Publication

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ARRDC1 (ARRDC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WZLneo-C/EBPα

Plasmid

#34567

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

C/EBPα (Cebpa Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD/HisD-LSSmKate2

Plasmid

#31909

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate2

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-V104M

Plasmid

#116363

Purpose

Lentiviral expression of ERBB3 V104M

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

V104M

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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IRAK1mut-UTR

Plasmid

#15096

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IRAK1 3'UTR (IRAK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Publication

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Dronpa-Lifeact-7

Plasmid

#54740

Purpose

Localization: Actin, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Publication

Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact-Dronpa

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSAC5.02

Plasmid

#16077

Depositor

Jochen Buck, Lonny Levin

Publication

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Expression

Mammalian

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET32a-HD25Q

Plasmid

#11508

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 25 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 25 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherryC2-Gephyrin P1

Plasmid

#68820

Purpose

mCherry tagged Geph for mammalian expression

Depositor

Shiva Tyagarajan

Publication

Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gephyrin (Gphn Rat)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Publication

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pBS IRES EGFP (DM#271)

Plasmid

#15080

Depositor

Douglas Melton

Publication

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

IRES EGFP

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only