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pDONR223_CTNNB1_p.S45F

Plasmid

#82821

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

S45F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R505C

Plasmid

#81507

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

R505C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP3K7_WT

Plasmid

#82321

Purpose

Gateway Donor vector containing MAP3K7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP3K7 (MAP3K7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLD_WT

Plasmid

#82326

Purpose

Gateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLD (CYLD Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_TRAF2_WT

Plasmid

#82239

Purpose

Gateway Donor vector containing TRAF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF2 (TRAF2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BCL2L1_WT

Plasmid

#82193

Purpose

Gateway Donor vector containing BCL2L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BCL2L1 (BCL2L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.T17N

Plasmid

#82179

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway Cloning

Mutation

T17N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_CBX3_WT

Plasmid

#82103

Purpose

Gateway Donor vector containing CBX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CBX3 (CBX3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EIF2A_WT

Plasmid

#82111

Purpose

Gateway Donor vector containing EIF2A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EIF2A (EIF2A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF6_WT

Plasmid

#82098

Purpose

Gateway Donor vector containing ATF6 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF6 (ATF6 Human)

Use

Gateway Cloning

Mutation

M67L; A145P; P157S; M313I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-HA

Plasmid

#79427

Purpose

Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(T27N)-HA

Plasmid

#79426

Purpose

Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 27 to Asparagine

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FKBP25-V5-APEX2

Plasmid

#83418

Purpose

Expressed FKBP25/FKBP3-V5-APEX2 in mammalian cell

Depositor

Hyun-Woo Rhee

Publication

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Peptidyl-prolyl cis-trans isomerase FKBP3 (FKBP3 Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1-C160S

Plasmid

#85666

Purpose

Expression of human YOD1 C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Publication

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YOD1 (YOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

C160S

Promoter

CMV

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Publication

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Publication

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pGAPTrap-mtagBFP2-IRESMuro

Plasmid

#82335

Purpose

Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Publication

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mtagBFP2-IRESMuro

Use

Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28-mTDGa.0

Plasmid

#81050

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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MLKL gRNA (BRDN0001146456)

Plasmid

#77540

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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