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pET-DEUT RUVBL1 Delete

Plasmid

#159144

Purpose

Expression and protien purification of mutated RUVBL1

Depositor

Eric Erquan Zhang

Publication

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL1 (RUVBL1 Human)

Expression

Bacterial

Mutation

amino acid 1-463, Glu134-Glu237 mutated to GPPG

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_2_T5

Plasmid

#155175

Purpose

see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Publication

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Publication

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMKO.1 puro-luc (shRNA)

Plasmid

#153962

Purpose

Expresses a shRNA against firefly luciferase

Depositor

Hiroyuki Konishi

Publication

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

shRNA against firefly luciferase (19-bp-long target)

Use

Luciferase, RNAi, and Retroviral

Tags

N/A

Expression

Mammalian

Promoter

U6 promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4 Cluster Promoter Delta 1.2

Plasmid

#100130

Purpose

It contains 0.5 Kb of the miR-183-96-182 cluster promoter. This fragment partially loses promoter activity in melanoma cells.

Depositor

Eva Hernando

Publication

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 0.5 Kb Fragment (MIR182 Human)

Use

Luciferase

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-Cluster Promoter Delta 1.1

Plasmid

#100129

Purpose

It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the minimal region that contains the maximal promoter activity

Depositor

Eva Hernando

Publication

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

miR-183-96-82 Cluster promoter 0.8 Kb Fragment (MIR182 Human)

Use

Luciferase

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Tags

Myc

Expression

Bacterial

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-FLAG-YWAA-pGK-HYG

Plasmid

#107375

Purpose

Lentiviral expression of FLAG-YWAA

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

Plasmid

#89890

Purpose

BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

HA-BirA-2A-citrine

Use

Unspecified

Tags

Citrine

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Publication

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-HT-Dmp53

Plasmid

#85698

Purpose

expression of Drosophila p53 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Publication

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Publication

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Publication

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Publication

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TT MEK5-DD-mRFP1

Plasmid

#47547

Purpose

Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Publication

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MEK5-DD (MAP2K5 Human)

Use

Gateway Cloning

Tags

mRFP

Mutation

S311D, T315D

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV-FFluc-GPD1L-3'UTR WT

Plasmid

#32488

Depositor

Pere Puigserver

Publication

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pAdEasy-EGFP-CLASP2 340-1084

Plasmid

#24524

Depositor

Torsten Wittmann

Publication

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only