19,830 results

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mEmerald-Fascin-C-10

Plasmid

#54094

Purpose

Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Fascin (FSCN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP WT

Plasmid

#123097

Purpose

Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Tags

GFP

Expression

Mammalian

Promoter

human PGK promoter

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-HD46Q

Plasmid

#11515

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 46Q glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 46 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC1

Plasmid

#153114

Purpose

Expression of Flag tagged human FOXC1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC1 (FOXC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6b

Plasmid

#66196

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6b

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLB1080

Plasmid

#37826

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

ATP7B (ATP7B Human)

Tags

mEGFP

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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plenti-CMV-mCherry-T2A-GFP

Plasmid

#109427

Purpose

A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-DUB3

Plasmid

#22593

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

DUB3 (USP17L9P Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CD2APtk

Plasmid

#86004

Purpose

homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

DsRedEx_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1

Plasmid

#27156

Purpose

For mammalian expression of human Elk-1 with an HA tag

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-ROC1

Plasmid

#20717

Depositor

Yue Xiong

Article

He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

Insert

RING finger protein 1 (ROC1) (RBX1 Human)

Tags

3xMyc

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgG4/κ

Plasmid

#61887

Purpose

Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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