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Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJBL3752

Plasmid

#128809

Purpose

Fluoride riboswitch-regulated sfGFP

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Su(fu) (CT#99)

Plasmid

#13856

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

GST

Expression

Bacterial

Mutation

Nco I fragment from full length; ~2kbp- ATG to st…

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pJJS012

Plasmid

#188335

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mTagBFP2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MYC-MST1

Plasmid

#8847

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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RP4-7

Plasmid

#79811

Purpose

Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Chloramphenicol acetyltransferase

Use

Helper plasmid for mobilization

Promoter

Own promoter

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-eIF4E

Plasmid

#232336

Purpose

Expresses human eIF4E (ENST00000450253.7, NM_001968.5) in mammalian cells

Depositor

Quanfu Ma

Insert

eIF4E (EIF4E Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-5X2-RalBP1

Plasmid

#37245

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

RalBP1 (RALBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7b

Plasmid

#51378

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7b (MIRLET7B Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-lyzC

Plasmid

#107591

Purpose

An entry vector with a lyzC promoter

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

Lysozyme C promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBbE2a-RFP

Plasmid

#35322

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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