20,361 results
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pcDNA3 Leo1
Plasmid#11061DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLeo1 (LEO1 Human)
ExpressionMammalianAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
Tags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet3-alphaTubulin
ExpressionMammalianPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE4
Plasmid#197148PurposeExpresses ApoE4 in Mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertApoE4 (APOE Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRatiometric pHLuorin2 fused to GPI anchor
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-GINKO2
Plasmid#177116PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2DepositorInsertGINKO2
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.617.2v1
Plasmid#179905PurposeEncodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationT19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…PromoterCAGAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Luc-noPS
Plasmid#177940PurposeTransient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuc2
ExpressionMammalianAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 300
Plasmid#163115PurposeRed fluorescent protein-based glucose sensor for mammalian cells with higer affinityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRed Glifon 300
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ABE8e-NRCH
Plasmid#165416PurposeExpresses the base editor ABE8e-NRCH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8e-NRCH
TagsBP-NLSExpressionMammalianPromoterCMVAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-HA-BACE1
Plasmid#165032PurposeHA-BACE1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBACE1 (BACE1 Human)
TagsHA (embedded after propeptide cleavage site)ExpressionMammalianPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
