We narrowed to 9,223 results for: Coli

1. pET mOCR His6 LIC cloning vector (2Oc-T)

   Plasmid

   #37238

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and mOCR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET MBP mCerulean LIC cloning vector (MBP-mCerulean)

   Plasmid

   #29751

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pColdII-SIRPT1-Latimeria chalumnae

   Plasmid

   #75356

   Purpose

   Nt His-tag Latimeria chalumnae Sacsin 94-1368

   Depositor

   Kalle Gehring

   Insert

   Sacsin Latimeria chalumnae 94-1368
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET mClover LIC cloning vector (u-mClover)

   Plasmid

   #86929

   Purpose

   pET LIC cloning vector for yORF-mClover

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   mClover

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pColdII-SIRPT1-Equus caballus

   Plasmid

   #78114

   Purpose

   Nt His-tag Equus caballus Sacsin 40-1317

   Depositor

   Kalle Gehring

   Insert

   Sacsin Equus caballus 40-1317
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET MBP mCitrine LIC cloning vector (MBP-mCitrine)

   Plasmid

   #29749

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pColdII-SIRPT1-Phoenix dactylifera

   Plasmid

   #78117

   Purpose

   Nt His-tag Phoenix dactylifera Sacsin 1-1295

   Depositor

   Kalle Gehring

   Insert

   Sacsin Phoenix dactylifera 1-1295
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pColdII-SIRPT1-Nelumbo nucifera

   Plasmid

   #75358

   Purpose

   Nt His-tag Nelumbo nucifera Sacsin 1-1313

   Depositor

   Kalle Gehring

   Insert

   Sacsin Nelumbo nucifera 1-1313
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK556

   Plasmid

   #74277

   Purpose

   Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-1246 to serine, numerous silent m…

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid

    #75323

    Purpose

    GST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376

    Depositor

    Kalle Gehring

    Insert

    Sacsin Xenopus (Silurana) tropicalis 87-1376
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pColdII-SIRPT1-Leptonychotes weddellii

    Plasmid

    #78115

    Purpose

    Nt His-tag Leptonychotes weddellii Sacsin 89-1367

    Depositor

    Kalle Gehring

    Insert

    Sacsin Leptonychotes weddellii 89-1367
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-SIRPT1-Ceratotherium simum simum

    Plasmid

    #75331

    Purpose

    GST fusion Ceratotherium simum simum Sacsin 89-1366

    Depositor

    Kalle Gehring

    Insert

    Sacsin Ceratotherium simum simum 89-1366
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-SIRPT1-Mustela putorius furo

    Plasmid

    #75333

    Purpose

    GST fusion Mustela putorius furo Sacsin 94-1381

    Depositor

    Kalle Gehring

    Insert

    Sacsin Mustela putorius furo 94-1381
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pColdII-SIRPT1-Sarcophilus harrisii

    Plasmid

    #75352

    Purpose

    Nt His-tag Sarcophilus harrisii Sacsin 90-1369

    Depositor

    Kalle Gehring

    Insert

    Sacsin Sarcophilus harrisii 90-1369
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColdII-SIRPT1-Serinus canaria

    Plasmid

    #75353

    Purpose

    Nt His-tag Serinus canaria Sacsin 90-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Serinus canaria 90-1368
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColdII-SIRPT1-Pelodiscus sinensis

    Plasmid

    #75354

    Purpose

    Nt His-tag Pelodiscus sinensis Sacsin 90-1379

    Depositor

    Kalle Gehring

    Insert

    Sacsin Pelodiscus sinensis 90-1379
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColdII-SIRPT1-Cucumis sativus

    Plasmid

    #75357

    Purpose

    Nt His-tag Cucumis sativus Sacsin 1-1292

    Depositor

    Kalle Gehring

    Insert

    Sacsin Cucumis sativus 1-1292
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pColdII-SIRPT1-Jatropha curcas

    Plasmid

    #75359

    Purpose

    Nt His-tag Jatropha curcas Sacsin 1-1304

    Depositor

    Kalle Gehring

    Insert

    Sacsin Jatropha-curcas 1-1304
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pColdII-SIRPT1-Chlorocebus sabaeus Sacsin

    Plasmid

    #78112

    Purpose

    Nt His-tag Chlorocebus sabaeus Sacsin 86-1375

    Depositor

    Kalle Gehring

    Insert

    Sacsin Chlorocebus sabaeus 86-1375
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIMcpf1

    Plasmid

    #153034

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKL003

    Plasmid

    #98919

    Purpose

    Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    PROPS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET24a isPETase

    Plasmid

    #225759

    Purpose

    Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    isPETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pXD70LacZ1-promoterless-LacZ

    Plasmid

    #191617

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSW068

    Plasmid

    #140036

    Purpose

    KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    p-T5 Lac

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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