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We narrowed to 18,759 results for: nls

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Showing: 1921 - 1960 of 18759 results

  10. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid
    #185706
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS
    Depositor
    Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Tags
    6xHis-NLS and NLS
    Expression
    Mammalian
    Mutation
    optimized for human codon usage
    Promoter
    CMV and T7
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185706-plasmid-map-sequence-id-363635

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  11. Ef1a-NLS-Gal4VP64TF-T2A-mCherry (MC) (pSS085)

    Plasmid
    #236353
    Purpose
    Second generation lentivirus compatible transfer vector encoding an GAL4 and an mCherry transduction marker
    Depositor
    Hana El-Samad
    Insert
    Ef1a-NLS-Gal4VP64TF-T2A-mCherry
    Use
    Lentiviral
    Promoter
    Ef1a
    Available since
    June 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    236353-plasmid-map-sequence-id-489665

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  13. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Publication
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

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  14. pRvCAHS1-mEGFP-NLS

    Plasmid
    #205013
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Publication
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205013-plasmid-map-sequence-id-405257

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  15. pRvSAHS1-mCherry-NLS

    Plasmid
    #205009
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Publication
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
    Use
    Tardigrade expression
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205009-plasmid-map-sequence-id-405252

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  16. JAB2603

    Plasmid
    #190390
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Publication
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
    Expression
    Plant
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190390-plasmid-map-sequence-id-393426

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  18. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194885
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Publication
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194885-plasmid-map-sequence-id-382817

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  19. pcDNA3-sACt-mCherry-NLS

    Plasmid
    #138214
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sACt-mCherry-NLS (Adcy10 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138214-plasmid-map-sequence-id-307416

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  20. pVE2_1_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1xQUAS

    Plasmid
    #154221
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 1xQUAS+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154221-plasmid-map-sequence-id-306061

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  21. pLM2_29_p40S_QfDBD_VP16AD_cp_nirA_DsRed_5xQUAS

    Plasmid
    #154235
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154235-plasmid-map-sequence-id-306060

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  22. pLM2_41_pgndA_QfDBD_VP16AD_cp_nirA_DsRed_5xQUAS

    Plasmid
    #154239
    Purpose
    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    pgndA:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    pGndA (An11g02040 A. niger)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154239-plasmid-map-sequence-id-306049

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  23. pLM2_36_p40S_QfDBD_Qf2AD_cp_pcbC_DsRed_5xQUAS

    Plasmid
    #154243
    Purpose
    Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-Qf2AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154243-plasmid-map-sequence-id-306039

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  24. pLM2_28_p40S_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS

    Plasmid
    #154236
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154236-plasmid-map-sequence-id-305990

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  25. pLM2_34_p40S_QfDBD_Qf2AD_cp_ura3_DsRed_5xQUAS

    Plasmid
    #154245
    Purpose
    Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-Qf2AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154245-plasmid-map-sequence-id-305988

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  26. pVE2_6_p40S_QfDBD_VP16AD_cp_penDE_DsRed_5xQUAS

    Plasmid
    #154233
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_penDE
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154233-plasmid-map-sequence-id-305986

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  27. pLM2_30_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

    Plasmid
    #154222
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154222-plasmid-map-sequence-id-305985

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  28. pVE2_5_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_5xQUAS

    Plasmid
    #154232
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbAB
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154232-plasmid-map-sequence-id-305984

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  29. pLM2_35_p40S_QfDBD_Qf2AD_cp_nirA_DsRed_5xQUAS

    Plasmid
    #154244
    Purpose
    Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-Qf2AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154244-plasmid-map-sequence-id-305983

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  30. pLM2_40_pgndA_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS

    Plasmid
    #154240
    Purpose
    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    pgndA:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    pGndA (An11g02040 A. niger)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154240-plasmid-map-sequence-id-305982

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  31. pVE2_7_p40S_QfDBD_VP16AD_cp_phl_DsRed_5xQUAS

    Plasmid
    #154234
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_phl
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    154234-plasmid-map-sequence-id-305895

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  32. pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS

    Plasmid
    #154225
    Purpose
    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    pgndA:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    pGndA (An11g02040 A. niger)
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    154225-plasmid-map-sequence-id-305810

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  34. pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

    Plasmid
    #244838
    Purpose
    Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus
    Depositor
    Jennifer Doudna
    Publication
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flex-Cas12a
    Use
    CRISPR
    Tags
    2xSV40 NLS
    Expression
    Mammalian
    Mutation
    G146R/R182V/D535G/S551F/D665N/E795Q
    Promoter
    T7
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244838-plasmid-map-sequence-id-486531

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  35. pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

    Plasmid
    #244842
    Purpose
    Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus
    Depositor
    Jennifer Doudna
    Publication
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    Flex-Cas12a
    Use
    CRISPR
    Tags
    4xSV40 NLS and 10xHis-CL7-2xSV40 NLS
    Expression
    Bacterial
    Mutation
    G146R/R182V/D535G/S551F/D665N/E795Q
    Promoter
    T7
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244842-plasmid-map-sequence-id-486525

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  38. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117168-plasmid-map-sequence-id-238529

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  39. PX458-3xHA-en15FnCas9

    Plasmid
    #201948
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xHA-NLS-en15FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201948-plasmid-map-sequence-id-405302

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Showing: 1921 - 1960 of 18759 results