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CL20_mEGFP_PAX3_FOXO1

Plasmid

#205875

Purpose

Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX3_FOXO1 (PAX3, FOXO1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSK

Plasmid

#136552

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKB

Plasmid

#23682

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2-T2A-Klf4-E2A-Myc

Plasmid

#210018

Purpose

episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2AV-p2a-KLF4

Plasmid

#193291

Purpose

episomal expressing human SOX2A61V + KLF4

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

CAG

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SKM

Plasmid

#136551

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2-p2a-KLF4

Plasmid

#193296

Purpose

episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK13

Plasmid

#20523

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK13 (MAPK13 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAPK7

Plasmid

#20527

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK7 (MAPK7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_AURKB_WT_V5

Plasmid

#82963

Purpose

Gateway Donor vector containing AURKB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AURKB (AURKB Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK13

Plasmid

#23739

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK13 (MAPK13 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
FUW-tetO-hOKMminiSOX

Plasmid

#206383

Purpose

Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Human, Mouse)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-ANKH_STOP

Plasmid

#161444

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

ANKH (ANKH Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-KMS

Plasmid

#188038

Purpose

Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OKM

Plasmid

#136554

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-KSM

Plasmid

#136553

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2-E2A-cMyc (Klf4, Sox2, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Beclin-1 [N248/32R]

Plasmid

#219461

Purpose

Mammalian Expression Plasmid of anti-Beclin-1 (Mouse) IgG2a R-mAb. Derived from hybridoma N248/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Beclin-1 (Mus musculus) recombinant mouse monoclonal antibody. (Becn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Glycine receptor Alpha3L [N424/48R]

Plasmid

#206574

Purpose

Mammalian Expression Plasmid of anti-Glycine receptor Alpha3L (Human). Derived from hybridoma N424/48.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Glycine receptor Alpha3L (Homo sapiens) recombinant Mouse monoclonal antibody (GLRA3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Glycine receptor Alpha3 [N424/45R]

Plasmid

#206573

Purpose

Mammalian Expression Plasmid of anti-Glycine receptor Alpha3 (Human). Derived from hybridoma N424/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Glycine receptor Alpha3 (Homo sapiens) recombinant Mouse monoclonal antibody (GLRA3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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