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We narrowed to 1,293 results for: Ncl

Showing: 961 - 980 of 1293 results
  1. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pMDC32B-AtMIR390a-NbSu-2

    Plasmid
    #213400
    Purpose
    Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor
    Depositor
    Insert
    Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
    Expression
    Plant
    Promoter
    2x35S
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Insert
    MSP1D1T2
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pET30-GBF-E6C

    Plasmid
    #21698
    Depositor
    Insert
    Protein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142) (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    Aug. 31, 2009
    Availability
    Academic Institutions and Nonprofits only
  7. enDr3

    Plasmid
    #241030
    Purpose
    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. 6xHis tag , thrombin cleavage site and 3xFLAG tag included in the fusion.
    Depositor
    Insert
    Ribonuclease HIII
    Tags
    3xFLAG and 6xHis-tag
    Expression
    Bacterial
    Mutation
    L52M
    Promoter
    T7
    Available Since
    June 8, 2026
    Availability
    Academic Institutions and Nonprofits only
  8. pETDuet-solo-NHis6-NocO-D282A

    Plasmid
    #240407
    Purpose
    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Asp…
    Promoter
    T7
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 961 - 980 of 1293 results