We narrowed to 5,510 results for: pcas

1. pCAG_Xph20-eGFP-CCR5TC

   Plasmid

   #135530

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_Xph20-GCaMP7f-CCR5TC

   Plasmid

   #216534

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   GCaMP7f

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_Xph20-mNeonGreen-CCR5TC

   Plasmid

   #135534

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_Xph15-mNeonGreen-CCR5TC

   Plasmid

   #135533

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_Xph20-SNAPf-CCR5TC

   Plasmid

   #135537

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   SNAPf

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG roGFP2-mito

   Plasmid

   #168509

   Purpose

   Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-roGFP2
   

   Use

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_Xph15-SNAPf-CCR5TC

    Plasmid

    #135536

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAM-kan-R4R3

    Plasmid

    #71275

    Purpose

    Binary destination vector for plant transformation. Kanamycin resistance.

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Type

    Empty backbone

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 spCas9-mSA

    Plasmid

    #113096

    Purpose

    pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    Synthetic Biology

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FLAG-mISG15

    Plasmid

    #12443

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Myr-p110-IH

    Plasmid

    #15689

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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