We narrowed to 6,573 results for: tac

1. GST-mDia1(FH1FH2DAD)-His

   Plasmid

   #85822

   Purpose

   contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

   Depositor

   Antoine Jégou, Guillaume Romet-Lemonne

   Article

   Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

   Insert

   Protein diaphanous homolog 1 (Diaph1 Mouse)
   

   Use

   Tags

   8*His and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-Rab8a-wt

   Plasmid

   #86078

   Purpose

   A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGST2-TSG101 (2-145)

   Plasmid

   #184810

   Purpose

   Bacterial expression of TSG101 UEV domain (aa 2-145)

   Depositor

   James Hurley

   Article

   Im et al Structure. 2010 Nov 10. 18(11):1536-47.

   Insert

   TSG101 (TSG101 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2-145 only

   Promoter

   tac

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPTF PHD and Bromodomain

   Plasmid

   #92101

   Purpose

   Expresses GST fusion PHD and Bromodomain from BPTF protein

   Depositor

   Albert Jeltsch

   Article

   Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

   Insert

   PHD and Bromodomain (BPTF Human)
   

   Use

   Tags

   GST Tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Grp1-PH pGEX2TK

   Plasmid

   #71379

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-2T_PV1_VP1

   Plasmid

   #202071

   Purpose

   Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   PV1 VP1 protein with Histag
   

   Use

   Tags

   6xHis-tag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

   Plasmid

   #222991

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T1-USP11

   Plasmid

   #78849

   Purpose

   To purify GST-tagged recombinant USP11 protein

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

   Insert

   USP11 (USP11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-parkin WT

    Plasmid

    #45969

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. HAI

    Plasmid

    #71316

    Purpose

    Inducible expression of human arginase I in E. coli

    Depositor

    David Christianson

    Article

    DiCostanzo et al Proc Natl Acad Sci U S A. 2005 Sep 13;10

    Insert

    human arginase I (ARG1 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGex-4T-2_GABARAP

    Plasmid

    #73948

    Purpose

    For recombinant expression of human GABARAP in E.coli

    Depositor

    Dieter Willbold

    Article

    Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGex-6p2_FANCM

    Plasmid

    #221379

    Purpose

    Expresses FANCM in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. polyPRM5

    Plasmid

    #175232

    Purpose

    Bacterial expression and purification, mixed with polySH3 to form condensates

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGex-6p2_ATRX

    Plasmid

    #221377

    Purpose

    Expresses ATRX in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1-Rac1 G15A

    Plasmid

    #69355

    Purpose

    expression of nucleotide free Rac1 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-6p-hDcp2

    Plasmid

    #72216

    Purpose

    bacterial expression of GST-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F146Y and E198K (please see depositor's comm…

    Promoter

    tac

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. HisMBP CFlm25

    Plasmid

    #78458

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie

    Article

    Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

    Insert

    Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

19. GST-GAR

    Plasmid

    #34697

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-145 of human fibrillarin with K2E and …

    Promoter

    tac

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

20. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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