We narrowed to 24,180 results for: CRISPR

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pFETCh_CEBPG

Plasmid

#86285

Purpose

Donor vector for 3' FLAG tag of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPG homology arms (CEBPG Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

Plasmid

#140201

Purpose

Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Cloning of crrna an…

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

Plasmid

#101205

Purpose

Bacterial expression plasmid for SpCas9 REC3 domain

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K506–Q712

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K506–Q712

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_GATAD2A

Plasmid

#86279

Purpose

Donor vector for 3' FLAG tag of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD2A homology arms (GATAD2A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.1

Plasmid

#78540

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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gh124

Plasmid

#106794

Purpose

expression of gRNA targeting ST8SIA4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA4 (ST8SIA4 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_MED13_iso1

Plasmid

#135733

Purpose

Donor vector for 3' FLAG tag of human MED13_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MED13_iso1 Homology arms (MED13 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106354

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMS6: pHelper(AvCAST)_entry_ΔTnsD

Plasmid

#168139

Purpose

Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8

Plasmid

#136376

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

Plasmid

#91920

Purpose

Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcsCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106352

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

Plasmid

#91913

Purpose

Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CaCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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gh87

Plasmid

#106758

Purpose

expression of gRNA targeting FUT3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT3 (FUT3 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106355

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

MYOD1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_BCL6_iso1

Plasmid

#104038

Purpose

Donor vector for 3' FLAG tag of human BCL6_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 homology arms (BCL6 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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