We narrowed to 13,211 results for: CAR

1. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

   Plasmid

   #113158

   Purpose

   An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV, CRISPR, and Cre/Lox

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #122103

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_BRD003 Luciferase

   Plasmid

   #117072

   Purpose

   single guide RNA targeting Luciferase

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   Luciferase
   

   Use

   CRISPR

   Promoter

   hU6

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

   Plasmid

   #84485

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α, 1.1 kb long (short version)

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv1-sgSHMT1-G3

   Plasmid

   #83903

   Purpose

   stable knockout

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Serine hydroxymethyltransferase 1 (SHMT1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR D-TOPO-C3_9

   Plasmid

   #60258

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   NKX6-1 enhancer (NKX6-1 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20_SLC38A2_wt

   Plasmid

   #156182

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23 C3_25

   Plasmid

   #60305

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ROCK1 enhancer (ROCK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

   Plasmid

   #114080

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_HA.AU1.VSVg_NGFR

    Plasmid

    #158232

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.AU1.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-PHB-4

    Plasmid

    #122231

    Purpose

    Expresses shRNA targeting the 3' UTR of human PHB

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    PHB (PHB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_NWS.AU1.VSVg_NGFR

    Plasmid

    #158239

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.AU1.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus1-DeltaCT-STAT3

    Plasmid

    #123178

    Purpose

    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    Deletion of the SH2 and TAD domains (C-terminus, …

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICE-EGFP-FLAG-TK1

    Plasmid

    #161919

    Purpose

    Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Thymidine kinase, cytosolic (TK1 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

    Plasmid

    #163178

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #118295

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEXSISERS_FLuc_loxP-PuroR

    Plasmid

    #177866

    Purpose

    Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    FLuc-based EXSISERS
    

    Use

    CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-AICD-NLS-IRES-hrGFP

    Plasmid

    #107543

    Purpose

    AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD-NLS (APP Human)
    

    Use

    AAV

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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