We narrowed to 17,309 results for: ESC

Showing: 9921 - 9940 of 17309 results

Results per page:

Sort results by:

pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Gira Bhabha, Damian Ekiert, Ian Henderson

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pACCS3-1

Plasmid

#78559

Purpose

4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.

Depositor

James Kaper

Article

Giron et al Vaccine. 1995 Jul;13(10):939-46.

Insert

cs3 operon

Expression

Bacterial

Promoter

tet

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pJB128 (MatP-mEos2)

Plasmid

#72653

Purpose

Inducible expression of MatP-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

MatP-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG 415

Plasmid

#63862

Purpose

Detect translation initiation from AUC start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUC start codon

Promoter

PtacI

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pSG 414

Plasmid

#63861

Purpose

Detect translation initiation from UUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 431

Plasmid

#63866

Purpose

Detect translation initiation from GUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

GUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pET-HRas-1-167-WT

Plasmid

#245306

Purpose

Expresses G-domain of human HRas (residues 1-167 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

HRas (HRAS Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 168-189

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)

Plasmid

#231130

Purpose

Expresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tyrosyl tRNA synthetase

Expression

Bacterial

Mutation

EcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

Plasmid

#240111

Purpose

A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added JeT Promoter

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pT/CAGGS-NRASG12V/D38A-IRES-mVenus

Plasmid

#236077

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V D38A (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V D38A

Promoter

CAGGS

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Orai1-jGCaMP8f V203Y

Plasmid

#231631

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f V203Y (ORAI1 Human, Synthetic)

Tags

jGCaMP8f V203Y

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Gluc-LHCGR

Plasmid

#221781

Purpose

Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

Depositor

Adolfo Rivero-Muller

Article

Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

Insert

LHCGR (LHCGR Human)

Tags

Gaussia Luciferase and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TH0901-pOCC177-FUS-wt_opt(Nhe)

Plasmid

#221885

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-REPORT (PGK) 12xHRE-NanoLuc

Plasmid

#208575

Purpose

hypoxic response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
12xHRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30b jGCaMP8f F366H

Plasmid

#204139

Purpose

Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Phenylalanine 366 changed to histidine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-REPORT(EF1a no GFP) mnuctdTomato

Plasmid

#172342

Purpose

mnuctdTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCytERM-hfYFP

Plasmid

#186520

Purpose

Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE1059

Plasmid

#186628

Purpose

Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbA
scbB
scbC

Use

Synthetic Biology

Tags

6xHis and 6xHis-thrombin

Expression

Bacterial

Promoter

Synthetic promoter B10, Synthetic promoter B19, a…

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge