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Showing: 9941 - 9960 of 65831 results

  1. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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  2. pAtVPZv1.0

    Plasmid
    #233147
    Purpose
    Expresses VDE, PsbS, ZEP from A.thaliana
    Depositor
    Krishna Niyogi
    Publication
    Kromdijk et al Science. 2016 Nov 18;354(6314):857-861.
    Inserts
    Resistance to herbicide bialaphos
    Zeaxanthin epoxidase (ABA1 Mustard Weed)
    Photosystem II subunit S (NPQ4 Mustard Weed)
    Violoxanthin de-epoxidase (NPQ1 Mustard Weed)
    Expression
    Plant
    Promoter
    FBA-2, GAPA-1, Rbcs1a, and mas promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233147-plasmid-map-sequence-id-462963

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  3. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

    Plasmid
    #108374
    Purpose
    Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Publication
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108374-plasmid-map-sequence-id-214321

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  4. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  5. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  6. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

    Plasmid
    #108373
    Purpose
    Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Publication
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108373-plasmid-map-sequence-id-208350

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  11. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47870
    Purpose
    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47870-plasmid-map-sequence-id-68843

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Showing: 9941 - 9960 of 65831 results