171,784 results
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Plasmid#127580PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2PromoterCMV immediate earlyAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialPromoterNoneAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1
Plasmid#1026DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-XPO1
Plasmid#116804PurposeLentiviral expression of XPO1DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1326
Plasmid#141811PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SeeSaw Reporter (SSR2.0)
Plasmid#50393PurposeSSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.DepositorInsertSSR2.0
ExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1159
Plasmid#144273PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citron1
Plasmid#134300PurposeBacterial expression of direct-response citrate sensorDepositorInsertCitron1
Tags6-His tagExpressionBacterialPromoterpBADAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
nesprin TSmod 25
Plasmid#125555PurposeNesprin-2G force sensor with cerulean-F25-venus TSmoduleDepositorInsertmini-nesprin-2G (Syne2 Mouse)
Tagscerulean, venusExpressionMammalianMutationTSmod changed from mTFP to cerulean, flageliform …PromoterCMVAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC53.2/PRMT5
Plasmid#35692DepositorInsertProtein arginine methyltransferase 5
PromoterSp6 for sense, T7 for sense and antisenseAvailable SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLXSN18E6E7
Plasmid#53459PurposeThe plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.DepositorInsertHPV18E6E7
UseRetroviralAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PASTE payload acceptor with attP site pDY2087
Plasmid#219859PurposeBxb1 payload vector with 5' attPDepositorInsertEGFP
UseCRISPRAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only