We narrowed to 2,493 results for: ERK

1. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Use

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-EKAR4

   Plasmid

   #174437

   Purpose

   Improved cyan/yellow FRET-based ERK kinase activity reporter.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-4511NES

   Plasmid

   #138375

   Purpose

   Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Booster-ERK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-endo-EKAR4

   Plasmid

   #205513

   Purpose

   Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-EKAR4
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MTK3_030

   Plasmid

   #123744

   Purpose

   Encodes ERK SPARK, a ERK activity reporter, as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERK SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R777-E125 Hs.MAPK1

   Plasmid

   #70409

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R777-E126 Hs.MAPK1-nostop

   Plasmid

   #70410

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pToneDest

   Plasmid

   #67691

   Purpose

   attR4-attR3 Destination cassette in Tol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pToneTP

   Plasmid

   #67692

   Purpose

   For in vitro transcription of mRNA encoding Tol1 Transposase

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   Tol1 transposase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AKARet-PSD

    Plasmid

    #114732

    Purpose

    PSD-targeted AKARet

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    PSD-targeted AKARet
    

    Use

    Tags

    EGFP and N-terminus of PSD-95

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. negEKARet-cyto

    Plasmid

    #114734

    Purpose

    Negative control of EKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of EKARet-cyto
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EKARet-PSD

    Plasmid

    #114723

    Purpose

    PSD-targeted EKARet

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    PSD-targeted EKARet
    

    Use

    Tags

    EGFP and N-terminus of PSD-95

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. negAKARet-cyto

    Plasmid

    #114733

    Purpose

    Negative control of AKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of AKARet-cyto
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Use

    Tags

    2A and 6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmTol-ins-UAS-R1R3-CC

    Plasmid

    #67745

    Purpose

    miniTol Destination vector with MAR-flanked UAS

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3xERRE-luciferase

    Plasmid

    #37851

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAPK1

    Plasmid

    #73248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AKARet-cyto

    Plasmid

    #96897

    Purpose

    FLIM sensor for PKA activation in cytosol

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for PKA Activity in Cytosol
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3EHA

    Plasmid

    #67708

    Purpose

    3' entry HA tag

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    HA tag
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS-Fv2Eflt4-2a-tdTomato

    Plasmid

    #67697

    Purpose

    Expression construct

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4-2A-tdTomato
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

21. 3xERRE/ERE-luciferase

    Plasmid

    #37852

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE/ERE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTiger-OptoSOS

    Plasmid

    #86439

    Purpose

    Single-construct blue light optogenetic Ras activator for use in Drosophila

    Depositor

    Jared Toettcher

    Article

    Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

    Insert

    OptoSOS
    

    Use

    Tags

    tagRFP

    Expression

    Insect

    Mutation

    Promoter

    Gal4-UAS

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p3E-2a-tdTomato

    Plasmid

    #67707

    Purpose

    3' entry 2A-tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    2A-tdTomato
    

    Use

    Tags

    Expression

    Mutation

    G172S (please see depositor's comments below)

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3E-2a-6MTnlstdTomato

    Plasmid

    #67699

    Purpose

    3' entry clone with 6xmyc nuclear localized tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    tdTomato
    

    Use

    Tags

    2A-6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTol1-UAS-nlstdTomato

    Plasmid

    #67693

    Purpose

    Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    6xMTnlsTomato
    

    Use

    Tags

    6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTol1-uas-mAKT-2a-nlstdTomato

    Plasmid

    #67706

    Purpose

    Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    mAKT-2A-nlstdTomato
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pME-6MTzactMap2k2b

    Plasmid

    #67710

    Purpose

    Middle entry clone

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated Map2k2b (map2k2b Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    S221D, S225D

    Promoter

    none

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dox-SOScat

    Plasmid

    #207329

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    son of sevenless homolog 2 variant, catalytic domain
    

    Use

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 593 to 1078

    Promoter

    Tet Response Element

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. miniTol2 x EF1a_EKAREN4 + PGK-blast

    Plasmid

    #167825

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR SFFVp YFP-PIF-SOS2cat

    Plasmid

    #50851

    Purpose

    Optogenetic Ras activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Inserts

    SOS2cat (SOS2 Human)
    PIF6 1-100
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mutation

    residues 566–1049

    Promoter

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR SFFVp BFP-PIF-SOS2cat

    Plasmid

    #50850

    Purpose

    Optogenetic Ras activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Inserts

    SOS2cat (SOS2 Human)
    PIF6 1-100
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    residues 566–1049

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pME-Fv2E-flt4um200-ns

    Plasmid

    #67696

    Purpose

    middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4um200
    

    Use

    Tags

    HA

    Expression

    Mutation

    um200, no stop codon; N1056S

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SPIB_pet28a

    Plasmid

    #131655

    Purpose

    Bacterial expression of ETS gene SPIB

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPIB (SPIB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SPDEF_pet28a

    Plasmid

    #131643

    Purpose

    Bacterial expression of ETS gene SPDEF

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPDEF (SPDEF Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SPIC_pet28a

    Plasmid

    #131654

    Purpose

    Bacterial expression of ETS gene SPIC

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPIC (SPIC Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV RhoB YFP MS2

    Plasmid

    #131777

    Purpose

    RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV Btg2 YFP MS2

    Plasmid

    #131776

    Purpose

    Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge