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Showing: 201 - 250 of 2321 results

  2. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Article
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    no
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59981-plasmid-map-sequence-id-96247

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  10. pEGFP-C1-M3(T3D)471-721

    Plasmid
    #56657
    Purpose
    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing
    Depositor
    Max Nibert
    Article
    Broering et al J Virol. 2005 May;79(10):6194-206.
    Insert
    M3(T3D)421-721
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    insert encodes aa 471-721 + native stop codon of …
    Promoter
    CMV-IE
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    56657-plasmid-map-sequence-id-87642

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  22. LIC-Z

    Plasmid
    #153543
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153543-plasmid-map-sequence-id-305901

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  26. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

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  28. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid
    #181872
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    Use
    AAV and Adenoviral
    Tags
    HA, T2A, and myc
    Expression
    Mammalian
    Mutation
    includes a large portion of the Slc8b1 3'UTR…
    Promoter
    synthetic hybrid CAG promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    181872-plasmid-map-sequence-id-356018

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  29. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  30. pOME0296M

    Plasmid
    #182625
    Purpose
    Reverse sequence of KSHV Kaposincluster-RevCompl-Syn for pcDNA
    Depositor
    Dirk Dittmer
    Insert
    KSHV Kaposincluster_RevCompl_SYN_rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182625-plasmid-map-sequence-id-359444

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  31. pJK683

    Plasmid
    #72378
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72378-plasmid-map-sequence-id-134010

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  32. pJK678

    Plasmid
    #72376
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72376-plasmid-map-sequence-id-138130

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  33. pAIP4_4HS5

    Plasmid
    #234223
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4HS5
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234223-plasmid-map-sequence-id-464188

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  34. pJK694

    Plasmid
    #72379
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  37. pCMV6-LacZ-BbsI

    Plasmid
    #114671
    Purpose
    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression
    Depositor
    Lynne Postovit
    Insert
    LacZ
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Lac promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    114671-plasmid-map-sequence-id-224108

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  38. pAIP4_1EW4

    Plasmid
    #234180
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EW4 (cyaY Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234180-plasmid-map-sequence-id-464145

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  39. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

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  40. pMDC32_PbGH3

    Plasmid
    #232335
    Purpose
    Generation of overexpressing Arabidopsis thaliana and Brassica napus lines to characterize the role of PbGH3 in plant.
    Depositor
    Edel Pérez-López
    Insert
    PbGH3
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232335-plasmid-map-sequence-id-463036

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  43. pDON-AI-baIaa

    Plasmid
    #51474
    Purpose
    Express mouse TH2B and TH2A in mammalian cells
    Depositor
    Shunsuke Ishii
    Inserts
    histone cluster 1, H2aa (H2ac1 Mouse)
    histone cluster 1, H2ba (H2bc1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51474-plasmid-map-sequence-id-78546

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  45. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  48. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

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Showing: 201 - 250 of 2321 results